Lihlahisoa

Tlhaloso:isoorientin ke inhibitor e sebetsang ea COX-2 e nang le boleng ba IC50 ba 39 μ M.

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Sepheo: cox-2:39 μ M (IC50)

Lithuto tsa in vitro:Isoorientin ke inhibitor e khethiloeng ea cyclooxygenase-2 (COX-2) ho tloha tuber ea Pueraria tuberosa [1].Lisele tsa PANC-1 le patu-8988 li ile tsa phekoloa ka Isoorientin (0,20,40,80 le 160 μ M) Hōla ka pel'a lihora tse 24 'me u kenye tharollo ea CCK8.Nakong ea 20, 40, 80 le 160 μ Nakong ea mahloriso a M, matla a lisele a fokotsehile haholo.Isoorientin (0,20,40,80 le 160) e ne e sebelisetsoa lisele μ M bakeng sa PANC-1;0, 20, 40, 80160 le 320 μ M e ne e sebelisetsoa setso sa patu-8988) bakeng sa lihora tse 24, 'me polelo ea P e ile ea hlahlojoa ke Western blot - AMPK le AMPK.Polelo ea p-ampk e ile ea eketseha ka mor'a phekolo ea Isoorientin.Joale, sehlopheng sa shRNA, 80 μ M mahloriso ho lemoha phello ea Isoorientin.Maemo a polelo a AMPK le p-ampk sehlopheng sa shRNA a ne a le tlase haholo ho feta a lisele tsa PC tsa mofuta o hlaha (WT) mme sehlopha se fetisitsoe ka lentivirus e mpe ea taolo (NC) [2].

Lithuto tsa in vivo:Liphoofolo tse tšoaroang ka Isoorientin ka 10 mg / kg le 20 mg / kg boima ba 'mele li bile le phokotso e kholo ea lipalo tsa claw edema, ka botenya ba tlhōrō ea 1.19 ± 0.05 mm le 1.08 ± 0.04 mm, ka ho latellana.Sena se bontšitse hore Isoorientin e fokolitse haholo paw edema ha e bapisoa le sehlopha sa taolo [3].

Teko ea lisele:Lisele tsa PANC-1 le tsa patu-8988 li ile tsa kenngoa lipoleiting tsa liliba tse 96.Seliba se seng le se seng se na le ~ lisele tse 5000 le lisele tse 200 μ L mahareng tse nang le 10% FBS.Ha lisele tsa seliba se seng le se seng li fihla ho 70% confluence, seaplane se ile sa fetoloa mme seaplane sa mahala sa FBS se nang le likhakanyo tse fapaneng tsa isoorientin e ile ea eketsoa.Ka mor'a lihora tsa 24, lisele li ne li hlatsuoa hanngoe ka PBS, setso sa setso se nang le isoorientin se ile sa lahloa, 'me 100% e ile ea eketsoa μ L FBS sebaka sa mahala le 10 μ L cell counting kit 8 (CCK8) reagent.Lisele li ile tsa kenngoa ka 37 ℃ bakeng sa lihora tse ling tsa 1-2, 'me ho kenngoa ha seliba ka seng ho ile ha fumanoa ho 490 nm ho sebelisa' mali oa ELISA.Ho sebetsa ha sele ho hlalosoa e le phetoho e ngata ea ho monya [2].

Teko ea liphoofolo:tabeng ea mohlala oa paw edema, litoeba [3] li ile tsa fuoa isoorientin kapa celecoxib intraperitoneally, 'me carrageenan e ne e kenngoa ka ho toba ka paw hora hamorao.Ka mohlala oa airbag, mekhoa eohle ea phekolo e kena ka mokotleng oa mokotla ka ho toba le carrageenan.isoorientin e ile ea kenngoa lihora tse 3 pele carrageenan e kenngoa ka har'a capsule.Isoorientin le celecoxib li ile tsa fuoa litoeba.Litharollo tsa stock tsa isoorientin (100 mg / ml) le celecoxib (100 mg / ml) li ile tsa lokisoa ka DMSO 'me tsa hlapolloa nakong ea kalafo.Liphoofolo li ne li arotsoe ka lihlopha tse hlano tse fapaneng tse latelang: taolo (DMSO tšoaroa);Carrageenan e phekotsoeng (0.5 ml (1.5% (w / V) carrageenan ka brine); e phekotsoe carrageenan + celecoxib (20mg / kg boima ba 'mele); Carrageenan + isoorientin e phekotsoeng (10 mg / kg boima ba 'mele); Phekola carrageenan + isoorientin (20mg / kg boima ba mmele).

Referense:[1].Sumalatha M, et al.Isoorientin, e Selective Inhibitor ea Cyclooxygenase-2 (COX-2) ho tloha Tubers ea Pueraria tuberosa.Nat Prod Commun.2015 Oct;10(10):1703-4.
[2].E, T, le ba bang.Isoorientin e etsa hore apoptosis e fokotsehe, e fokotse ho hlasela, 'me e theole secretion ea VEGF ka ho kenya tšebetsong pontšo ea AMPK liseleng tsa mofetše oa pancreatic.Onco Targets Ther.2016 Dec 12;9:7481-7492.
[3].Anilkumar K, le ba bang.Tlhahlobo ea Thepa ea Anti-Inflammatory ea Isoorientin e Isolated ho tloha Tubers ea Pueraria tuberosa.Oxid Med Cell Longev.2017;2017:5498054.