Lihlahisoa

Sepheo:JNK1 jnk2 NF- κ B

Thuto ea in vitro:isovitexin e thibela tšenyo ea oxidative e bakoang ke LPS ka ho thibela tlhahiso ea intracellular ROS, hape e fokolisa phello ea H2O2 ho sebetsa ha sele.E na le LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) e ne e se na cytotoxicity ho lisele tse tala tsa 264.7, empa 200 μ G / ml isovitexin e bontšitse cytotoxicity ea bohlokoa.Isovitexin (25,50) μ G / ml) e thibela LPS e kentse TNF- α, Ho eketseha ha maemo a IL-6, iNOS le COX-2.Isovitexin (25,50) μ G / ml) e boetse e thibela I ka lisele tse tala tsa 264.7 κ B α Phosphorylation le ho senyeha, e lumellanang le phello ea JNK1 / 2 inhibitor [1].

Lithuto tsa in vivo:isovitexin (50 le 100 mg / kg, IP) e bakile liphetoho tse fokolang haholo tsa histopathological likarolong tsa matšoafo le ho fokotsa palo ea lisele tsa ho ruruha ho litoeba tse susumelitsoeng ke LPS.Heteroeosinophils (50 le 100 mg / kg, IP) e fokotsehile TNF ka- α Le tlhahiso ea IL-6, tlhahiso ea ROS, MPO le MDA content, ho eketseha ha SOD le GSH, le ho thibela LPS ho baka ho ruruha le khatello ea oxidative LPS e entseng litoeba tsa Ali.'Me ka katleho e thibela polelo ea protheine ea iNOS le COX-2 [1].Isovitexin (25,50, 100 mg / kg) e fokolitse sekhahla sa ho pholoha ha kotsi ea sebete e bakoang ke LPS / D-gal ho litoeba ka mokhoa o itšetlehileng ka tekanyetso.Isovitexin e boetse e thibela NF- κ B le holimo e laola LPS / D-gal e entsoeng Nrf2 le HO-1 ho litoeba [2].

Teko ea lisele:ho sebetsa ha sele ho ile ha khethoa ke tlhahlobo ea MTT.Lisele tse tala tsa 264,7 li ne li kenngoa lipoleiti tsa seliba tse 96 (1) × 104 lisele / seliba) 'me li na le mefuta e sa tšoaneng ea isovitexin (mahloriso a ho qetela: 0-200) μ G / ml) le LPS (2 μ G / ml) bakeng sa lihora tse 24.Ho phaella moo, sebelisa IV (25 kapa 50 μ G / ml) pretreated lisele bakeng sa hora e le 1, 'me joale H 2O 2 (300) e ile ea eketsoa μ M). Ka mor'a lihora tse 24, MTT (5 mg / ml) e ile ea eketsoa ho disele di bo di elama diura di le 4 [1].

Teko ea liphoofolo:litoeba [1] e le ho theha mohlala oa Ali, litoeba li ne li arotsoe ka lihlopha tsa 6: taolo (saline), feela isovitexin (100 mg / kg, e qhibilihisitsoeng ka 0.5% DMSO), feela LPS (0.5 mg / kg, e qhibilihisitsoeng ka letsoai). ), LPS (0.5 mg / kg) + isovitexin (50 kapa 100 mg / kg) le LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, e qhibilihisitsoeng ka letsoai).Isovitexin kapa DEX (5 mg / kg) isovitexin e ne e tsamaisoa.Ka mor'a ho pepesehela isovitexin kapa DEX bakeng sa hora ea 1, litoeba li ne li kokobetsoa ka ether 'me LPS e ne e tsamaisoa ka intranasally (in) ho baka kotsi ea matšoafo.Liphoofolo li ile tsa hlakoloa lihora tse 12 kamora tsamaiso ea LPS.Ka hona, bronchoalveolar lavage fluid (BALF) le lisampole tsa lisele tsa matšoafo li ile tsa kotuloa ho lekanya maemo a cytokine;moloko oa ROS;Mesebetsi ea SOD, GSH, MDA le MPO;Le polelo ea COX-2, iNOS, HO-1 le liprotheine tsa Nrf2 [1].