Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
Boitsebiso ba Bohlokoa
Salvianolic acid B ke condensation ea limolek'hule tse tharo tsa Danshensu le molek'hule e le 'ngoe ea caffeic acid.Ke e 'ngoe ea li-salvianolic acid tse ithutoang haholo.E na le litlamorao tsa bohlokoa tsa pharmacological ho pelo, boko, sebete, liphio le litho tse ling.Sehlahisoa sena se na le litlamorao tsa ho khothaletsa phallo ea mali le ho tlosa stasis ea mali, ho hula li-meridians le ho kenya li-collaterals.Haholo-holo e sebelisoa ho phekola lefu la ischemic le bakoang ke mali a thibelang meridians, joalo ka ho tsieleha ha halofo ea 'mele le maoto le matsoho, bofokoli, bohloko ba konteraka, ho hloleha ha motlakase, ho kheloha ha molomo le mahlo, joalo-joalo.
Leetsi:salvianolic acid B, salvianolic acid B, salvianolic acid B
Lebitso la Senyesemane:salvianolic acid B
Foromo ea molek'hule:c36h30o16
Boima ba molek'hule:718.62
Nomoro ea CAS:115939-25-8
Mokhoa oa ho lemoha:HPLC ≥ 98%
Litlhaloso:10mg, 20mg, 100mg, 500mg, 1g (e ka pakoa ho latela litlhoko tsa bareki)
Mosebetsi le tšebeliso:Sehlahisoa sena se sebelisetsoa ho tseba litaba.
Lintho tsa 'mele le tsa lik'hemik'hale
Thepa:sehlahisoa ke quasi e tšoeu phofo.
Tatso e baba hanyenyane ebile e astringent, e na le thepa e kenyang mongobo.E qhibiliha ka metsing, ethanol le methanol.
Salvianolic acid B e entsoe ka condensation ea limolek'hule tse 3 tsa salvianolic acid le molek'hule e le 'ngoe ea caffeic acid.E na le lihlopha tse peli tsa carboxyl mme e teng ka mokhoa oa letsoai le fapaneng (K +, Ca2 +, Na +, NH4 +, joalo-joalo).Nakong ea Decoction le mahloriso, karolo e nyenyane ea salvianolic acid B e hydrolyzed ho pherese oxalic acid le salvianolic acid, 'me karolo ea salvianolic acid B e fetoha acid e rosmarinic tlas'a maemo a acidic;Salvianolic acid A le C e ka ba tautomeric ka tharollo.
Litlhaloso
5%, ~ 10%, ~50%, ~70%, ~90%,98%
Mokhoa oa ho Ntša
Radix Salviae Miltiorrhizae e ile ea pshatleha, ea kenngoa ka tankeng e ntšoang, ea kolobisoa ka makhetlo a 8 ho feta 0.01mol/l hydrochloric acid ka bosiu bo le bong, eaba e tšeloa ka makhetlo a 14 ho feta palo ea metsi.Tharollo e ntšitsoeng e ntšitsoeng e hloekisoa ke kholomo ea AB-8 macroporous resin.Ntlha ea pele, e-lute e nang le 0.01mol/l hydrochloric acid ho tlosa litšila tse sa adsorbed, ebe e fana ka 25% ea ethanol ho tlosa litšila tse ngata tsa polar.Qetellong, tsepamisa maikutlo a 40% ea ethanol tlas'a khatello e fokotsehileng ea ho khutlisa ethanol le ho omella ho fumana kakaretso ea Salvia miltiorrhiza phenolic acid ka bohloeki bo fetang 80%.
Tseba
Nka 1g ea sehlahisoa, u e sile, eketsa 5ml ea ethanol, hlohlelletsa ka ho feletseng, sefa, nka marotholi a seng makae a filtrate, u e behe holim'a pampiri ea pampiri, e omise, e shebe tlas'a lebone la ultraviolet (365nm), bonts'a blue- grey fluorescence, fanyeha pampiri ea filthara ka har'a botlolo ea tharollo ea ammonia e tsitsitseng (e sa kopane le metsi), e ntše ka mor'a metsotso e 20, e shebe tlas'a lebone la ultraviolet (365nm), e bonts'a fluorescence e putsoa-botala.
Asiti:nka tharollo ea metsi tlas'a ntlha ea ho hlaka, 'me boleng ba pH e tla ba 2.0 ~ 4.0 (sehlomathiso sa Khatiso ea Pharmacopoeia 1977 ea Chaena).
Boikemisetso ba Litaba
E ile ea khethoa ke chromatography ea metsi a phahameng a sebetsang (Sehlomathiso VI D, Volume I, Chinese Pharmacopoeia, 2000 EDITION).
Octadecyl silane bonded silica gel e ne e sebelisoa e le filler maemong a chromatographic le tlhahlobo ea ts'ebetso ea sistimi;Methanol acetonitrile formic acid water (30:10:1:59) e ne e le mohato o tsamaeang;Nako ea ho lemoha e ne e le 286 nm.Palo ea lipoleiti tsa theoretical tse baloang ho latela salvianolic acid B tlhorong ha ea lokela ho ba ka tlase ho 2000.
Ho lokisoa ha tharollo ea litšupiso ho lekanya ka nepo palo e nepahetseng ea tharollo ea sesupo sa salvianolic acid B le ho eketsa metsi ho etsa hore e be le 10% ka 1ml μ G tharollo.
Ho lokisoa ha tharollo ea teko ho nka hoo e ka bang 0.2g ea sehlahisoa, ho e lekanya ka nepo, e kenye ka botlolong ea ho lekanya 50ml, eketsa palo e loketseng ea methanol, sonicate metsotso e 20, e pholise, eketsa metsi sekaleng, e sisinye hantle, sefa. eona, ka nepo lekanya 1ml ea filtrate e tsoelang pele, e kenye ka botlolo ea ho lekanya 25ml, eketsa metsi sekala, e sisinye hantle.
Mokhoa oa ho etsa qeto ka nepo o nka 20% ea tharollo ea taolo le 20% ea tharollo ea teko μ l.E kenye ka har'a mokelikeli oa chromatograph bakeng sa ho tseba.
Pharmacological Efficacy
Salvianolic acid B ke condensation ea limolek'hule tse tharo tsa Danshensu le molek'hule e le 'ngoe ea caffeic acid.Ke e 'ngoe ea li-salvianolic acid tse ithutoang haholo.E na le litlamorao tsa bohlokoa tsa pharmacological ho pelo, boko, sebete, liphio le litho tse ling.
Antioxidant
Salvianolic acid B e na le phello e matla ea antioxidant.Liteko tsa vivo le in vitro li bonts'a hore salvianolic acid B e khona ho tlosa li-radicals tsa mahala tsa oksijene le ho thibela lipid peroxidation.Matla a ts'ebetso ea eona a phahame ho feta a vithamine C, vithamine E le mannitol.Ke e 'ngoe ea lihlahisoa tse tsebahalang tsa tlhaho tse nang le phello e matla ea antioxidant Liphuputso tsa Pharmacological li bontša hore salvianolic acid bakeng sa ente e na le phello e hlakileng ea antioxidant, e thibela ho bokellana ha platelet le thrombosis,' me e ka lelefatsa nako ea ho phela ha liphoofolo tlas'a hypoxia.Liphello li bontšitse hore salvianolic acid bakeng sa ente (60 ~ 15mg / kg) e ka ntlafatsa haholo khaello ea methapo ho likhoto tse nang le kotsi ea cerebral ischemia-reperfusion, ho ntlafatsa boits'oaro le ho fokotsa haholo sebaka sa cerebral infarction.Ho ne ho e-na le phapang e khōlō pakeng tsa litekanyetso tse phahameng le tse mahareng (60 le 30mg / kg);Salvianolic acid bakeng sa ente e ka ntlafatsa haholo tšenyo ea methapo e bakiloeng ke FeCl3 e bakileng cerebral ischemia ho likhoto ka 1, 2 le lihora tsa 24 ka mor'a tsamaiso, e bonahatsoang ka ntlafatso ea bothata ba boitšoaro le ho fokotsa sebaka sa cerebral infarction;Salvianolic acid 40 mg / kg bakeng sa ente e ile ea thibela haholo ho bokellana ha liplatelete tsa mmutla tse hlahisoang ke ADP, arachidonic acid le collagen, 'me litekanyetso tsa ho thibela e ne e le 81.5%, 76.7% le 68.9% ka ho latellana.Salvianolic acid 60 le 30mg / kg bakeng sa ente e thibela haholo thrombosis ho likhoto;Salvianolic acid 60 le 30mg / kg bakeng sa ente e lelefatsa nako ea ho phela ea litoeba tlas'a hypoxia haholo.
Kopo ea Clinical
Sehlahisoa sena se na le litlamorao tsa ho khothaletsa phallo ea mali le ho tlosa stasis ea mali, ho hula li-meridians le ho kenya li-collaterals.Haholo-holo e sebelisoa ho phekola lefu la ischemic le bakoang ke mali a thibelang meridians, joalo ka ho tsieleha ha halofo ea 'mele le maoto le matsoho, bofokoli, bohloko ba konteraka, ho hloleha ha motlakase, ho kheloha ha molomo le mahlo, joalo-joalo.
Lebenkele
Sebakeng se phodileng le se omileng.
Nako ea ho Tiea
Lilemo tse peli.
Mokhoa oa ho boloka
2-8 ° C, e bolokiloe sebakeng se pholileng le se omileng le hole le leseli.
Litaba Tse Lokelang ho Eloa Hloko
Sehlahisoa se lokela ho bolokoa ka mocheso o tlase.Haeba e pepesehetse moea ka nako e telele, litaba li tla fokotseha.
