Lihlahisoa

Typhonine glycoside

Puhuang ke peo e phofshoana e omisitsoeng ea Typha angustifolia L., Typha Orientalis Presl kapa limela tse tšoanang le tsena ho Typhaceae.Likahare tsa isorhamnetin-3-o-neohesperidin ho Chinese Pharmacopoeia (Khatiso ea 2005) li khethoa ke HPLC.Mekhoa e ka sehloohong ea ho etsa qeto ea isorhamnetin-3-o-neohesperidin ho Typhae le litokisetso tsa eona ke

HPLC.Tlas'a boikemisetso ba litaba tsa Typha in Chinese Pharmacopoeia (Khatiso ea 2005):

Maemo a Chromatographic le tlhahlobo ea ts'ebeliso ea sistimi: octadecyl silane bonded silica gel e le filler;Metsi a acetonitrile (15:85) a ne a sebelisoa e le mohato o tsamaeang;Nako ea ho lemoha e ne e le 254 nm.Mocheso oa kholomo 40 ℃.Palo ea lipoleiti tsa theoretical ha e na ho ba tlase ho 1000 ho ea ka tlhoro ea isorhamnetin-3-o-neohesperidin.

Tokisetso ea tharollo ea teko: nka hoo e ka bang 0.5g ea sehlahisoa sena, se lekanya ka nepo, se kenye ka botlolong ea ho lekanya 50ml, eketsa 45ml ea methanol, kalafo ea ultrasonic (matla a 250W, frequency 20KHz) metsotso e 30, e pholise, eketsa methanol ho sekaleng, e sisinye hantle, e sefa, 'me u nke filtrate e tsoelang pele

Boikemisetso ba isorhamnetin-3-o-neohesperidin e Puhuang le litokisetso tsa eona ke HPLC.Sistimi e akaretsang ea mobile phase ke metsi a acetonitrile:

① Hp1050 chromatograph ea mokelikeli o phahameng oa ts'ebetso e phahameng;Kholomo ea Chromatographic C18 (5) μ m,4.6mm × 250mm) Setsi sa Dalian sa Chemical Physics, Setsi sa Setsi sa Sechaena sa Saense);Mokhahlelo oa selefouno: metsi a acetonitrile (15:85);Leqhubu la ho lemoha: 254nm;Kholomo mocheso oa sethopo.

② Mokelikeli oa chromatograph o sebetsang ka holimo ke hp1050 tsamaiso ea Hewlett Packard Company United States;Chromatographic column bondclone 10 C18 (3.9mm) × 300mm）; Mokhahlelo oa selefouno: metsi acetonitrile (80:20);ho lemoha wavelength 254 nm, bandwidth 4 nm;Reference wavelength 550 nm, bandwidth 100 nm;nyenyefatso 8.

Fu Daxu et al.E ile ea etsa qeto ea litaba tsa typhoniflorin le isorhamnetin-3-o-neohesperidin ka kakaretso ea flavone e ntšitsoeng ea Typha ka HPLC le 1100 chromatograph.Kholomo ea chromatographic ke eclipse XdB C18 (250mm × 4.6mm，5 μ m); Mohato oa selefouno: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15:78:7; 15min: 23:70:7);Leqhubu la ho lemoha ke 248 nm.Tokisetso ea kakaretso ea flavonoids e ntšitsoeng ea Typha: 50g ea Typha e ne e ntšitsoe ka 60% ethanol (500ml) × 2), 1H nako le nako, motsoako o ne o tsepamisitsoe ho 40 ℃, motsoako o ne o qhibiliha ka metsi a 600ml le centrifuged.Masala a ile a qhibiliha ka 300ml ea metsi le centrifuged.Kopanya tharollo centrifugal, lokisa bophahamo ba modumo ho 1000ml ka metsi, adsorb e holim'a kholomo ea macroporous resin (150ml macroporous resin dry boima 75g) le 1.5 linako tse ngata tsa lithethefatsi tse tala, elute le 600ml ea metsi le 450ml ea 20 ho tlosa ethanol litšila, joale elute le 600ml ea 50% ethanol, le eluent e fumanoa ka vacuum omisa ka 40 ℃.

Yang Yonghua et al.E sebelisitsoeng ts'ebetso e phahameng ea capillary electrophoresis le ts'ebetso e phahameng ea mokelikeli chromatography ho fumana litaba tsa isorhamnetin 232o2, neohesperidin le typhoside Puhuang.Hpce-3d high performance capillary electrophoresis instrument (Hewlett Packard, USA), quartz capillary column 50 μ m × 56cm (HP), lc-6a high performance liquid chromatograph (Shimadzu company of Japan), C18 column 5mm × 200mm, particle size 5 μ M (Khamphani ea Dalian Yite).Maemo a HPCE: buffer 0.02mol/l borax-0.05mol/l 10% acetonitrile ea sodium dodecyl sulfate (SDS), voltage 20kV, kholomo mocheso 30 ℃, ho lemoha wavelength 270nm, ente bophahamo ba modumo 30KPa · s.Maemo a HPLC: karolo ea selefouno e ne e le metsi a acetonitrile (15:85), mocheso oa kholomo e ne e le 25 ℃, 'me bolelele ba ho lemoha e ne e le 254 nm.

Ntho ea typhaneoside Reference

[lebitso]typhonioside [1]

[Nomoro ea CAS]104472-68-6

[mokhoa oa ho lemoha]HPLC ≥ 98%

[Tlhaloso]20mg 50mg 100mg 500mg 1g (e ka pakoa ho latela litlhoko tsa bareki)

[thepa]phofo ea amorphous