Lihlahisoa

Thuto ea In Vitro:

Joalo ka tlholisano ea PKC Inhibitor ea ATP, Verbascoside e na le IC50 ea 25 μ M. Verbascoside e bontšitse kis ea 22 le 28 mabapi le ATP le histone, ka ho latellana μ M [1]。 Verbascoside (5,10) μ M) Thibelo ea 2,4-dinitrochlorobenzene (DNCB) - induced T cell costimulatory factor CD86 le CD54, proinflammatory cytokines le NF ka thk-1 lisele κ B tsela activation [2].

Lithutong tsa Vivo:

Verbascoside (1%) e fokolitse ts'ebetso ea boits'oaro ka kakaretso le ho teba ha liso tsa letlalo ka mohlala oa toeba ea 2,4-dinitrochlorobenzene (DNCB) - induced atopic dermatitis (AD).Verbascoside e ka boela ea thibela pro-inflammatory cytokine TNF ho DNCB e bakoang ke liso tsa letlalo- α, Tlhaloso ea IL-6 le IL-4 mRNA [2].Verbascoside (50100 mg / kg, IP) ha ea ka ea fetola bohloko bo batang bo sa tloaelehang bo bakoang ke kotsi e sa foleng ea khatello (CCI).Verbascoside(200 mg / kg, IP) e fokolitse ho hanyetsa ho bata ho susumetsoang ke acetone letsatsing la 3. Verbascoside e boetse e fokotsa haholo liphetoho tsa boitšoaro tse amanang le lefu la methapo.Ho phaella moo, Verbascoside e ile ea fokotsa Bax 'me ea eketseha Bcl-2 ka letsatsi la 3 [3].

Teko ea Cell:

lymphocytic mouse leukemia L1210 cells (ATCC, CCL 219) e ne e na le 10% fetal bovine serum, 4 mM glutamine, 100 U / ml penicillin, 100 μ Ka har'a poleiti ea seliba sa 24 ea Dulbecco e fetotsoeng bohareng ba Ntsu, lisele tse 104 seliba ka seng li ne li fokola. Ml streptomycin sulfate le Verbascoside (e qhibilihisitsoeng ka DMSO).Khōlo e ile ea hlokomeloa ka ho bala palo ea lisele tse ka har'a counter ea Coulter ka mor'a matsatsi a 2 a ho kenngoa sebakeng se mongobo (5% CO2 moeeng) ho 37 ℃.Boleng ba IC50 bo ne bo baloa ho ipapisitsoe le mohala oa ho theola moeli o thehiloeng bakeng sa motsoako o mong le o mong oa teko [1].

Teko ea Liphoofolo:

E le ho etsa hore atopic dermatitis (AD) - joalo ka matšoao, likhoto [2] li sebelisitse 2,4-dinitrochlorobenzene (DNCB).Ka bokhutšoanyane, moriri oa mokokotlo oa litoeba o ile oa tlosoa ka lisekere tsa elektronike matsatsi a 2 pele ho phekolo ea DNCB.Will 200 μ L ea 1% DNCB (ka acetone: oli ea mohloaare = 4: 1) e ne e sebelisoa ho beola letlalo la mokokotlo bakeng sa ho susumetsa.Litlhaselo tse pheta-phetoang li ile tsa etsoa sebakeng se le seng, 0.2% DNCB matsatsi a mang le a mang a 3 bakeng sa libeke tse ka bang 2.Litoeba li arotsoe ka lihlopha tsa 4 (n = 6 sehlopheng ka seng): (1) taolo ea phekolo ea koloi, (2) DNCB e tšoaroa feela, (3) 1% Verbascoside (acetone: oli ea mohloaare 4: 1) - e tšoaroa feela, le ( 4) DNCB + 1% Sehlopha sa phekolo ea Verbascoside[2].

Referense:

[1].Herbert JM, le ba bang.Verbascoside e arohaneng le Lantana camara, inhibitor ea protein kinase C. J Nat Prod.1991 Nov-Dec;54(6):1595-600.

[2].Li Y, le ba bang.Verbascoside e Fokotsa Matšoao a Atopic Dermatitis-Joaloka Litoeba ka Phello ea Eona e Matla ea Anti-Inflammatory.Int Arch Allergy Immunol.2018;175(4):220-230.

[3].Amin B, le ba bang.Phello ea Verbascoside ho Neuropathic Pain E Bakiloeng ke Kotsi e sa Feleng ea Constriction ho Likhoto.Phytother Res.2016 Jan;30(1):128-35.