Verbascoside CAS No. 61276-17-3
Essential Information
[name] Mullein glycoside
[alias] ergosterol, Mullein
[category] phenylpropanoid glycosides
[English name] acteoside; Verbascoside; Kusaginin
[molecular formula] C29H36O15
[molecular weight] 624.59
[CAS No.] 61276-17-3
Physicochemical Properties
[properties] this product is white needle crystal powder
[relative density] 1.6g/cm3
[solubility] easily soluble in ethanol, methanol and ethyl acetate.
Extraction Source
This product is the dry fleshy stem with scaly leaves of Cistanche deserticola, a plant of liedang family.
Test Method
HPLC ≥ 98%
Chromatographic conditions: mobile phase methanol acetonitrile 1% acetic acid (15:10:75), flow rate 0.6 ml · min-1, column temperature 30 ℃, detection wavelength 334 nm (for reference only)
Function and Use
This product is used for content determination
Storage Method
2-8 ° C, stored away from light.
Bioactivity of Verbascoside
In Vitro Study:
As a competitive PKC Inhibitor of ATP, Verbascoside has an IC50 of 25 μ M。 Verbascoside showed kis of 22 and 28 relative to ATP and histone, respectively μ M。 Verbascoside has effective antitumor activity on L-1210 cells with IC50 of 13 μ M[1]。 Verbascoside (5,10) μ M) Inhibition of 2,4-dinitrochlorobenzene (DNCB) - induced T cell costimulatory factors CD86 and CD54, proinflammatory cytokines and NF in thk-1 cells κ B pathway activation [2].
In Vivo Studies:
Verbascoside (1%) reduced the incidence of overall scratching behavior and the severity of skin lesions in a mouse model of 2,4-dinitrochlorobenzene (DNCB) - induced atopic dermatitis (AD). Verbascoside can also block the pro-inflammatory cytokine TNF in DNCB induced skin lesions- α， Expression of IL-6 and IL-4 mRNA [2]. Verbascoside (50100 mg / kg, IP) did not change the cold abnormal pain caused by chronic compressive injury (CCI). Verbascoside(200 mg / kg, IP) reduced allergy to cold stimulated acetone on day 3. Verbascoside also significantly reduced the behavioral changes associated with neuropathy. In addition, Verbascoside reduced Bax and increased Bcl-2 on day 3 [3].
Cell Experiment:
lymphocytic mouse leukemia L1210 cells (ATCC, CCL 219) contained 10% fetal bovine serum, 4 mM glutamine, 100 U / ml penicillin, 100 μ In the 24 well cluster plate of Dulbecco modified Eagle medium, 104 cells per well were sparsely laid/ Ml streptomycin sulfate and Verbascoside (dissolved in DMSO). Growth was monitored by counting the number of cells in the Coulter counter after 2 days of incubation in a humid atmosphere (5% CO2 in air) at 37 ℃. The IC50 value was calculated based on the linear regression line established for each test compound [1].
Animal Experiment:
In order to induce atopic dermatitis (AD) - like symptoms, rats [2] used 2,4-dinitrochlorobenzene (DNCB). In short, the dorsal hair of mice was removed with electronic scissors 2 days before DNCB treatment. Will 200 μ L of 1% DNCB (in acetone: olive oil = 4:1) was applied to shaved back skin for sensitization. Repeated attacks were performed at the same site, 0.2% DNCB every 3 days for about 2 weeks. Mice were divided into 4 groups (n = 6 in each group): (1) vehicle treated control, (2) DNCB treated only, (3) 1% Verbascoside (acetone: olive oil 4:1) - treated only, and (4) DNCB + 1% Verbascoside treated group[2].
Reference:
[1]. Herbert JM, et al. Verbascoside isolated from Lantana camara, an inhibitor of protein kinase C. J Nat Prod. 1991 Nov-Dec;54(6):1595-600.
[2]. Li Y, et al. Verbascoside Alleviates Atopic Dermatitis-Like Symptoms in Mice via Its Potent Anti-Inflammatory Effect. Int Arch Allergy Immunol. 2018;175(4):220-230.
[3]. Amin B, et al. The Effect of Verbascoside in Neuropathic Pain Induced by Chronic Constriction Injury in Rats. Phytother Res. 2016 Jan;30(1):128-35.
