I-Astragaloside IV CAS No. 84687-43-4
Intshayelelo emfutshane
Igama lesiNgesi:I-Astragaloside IV;i-beta-D-Glucopyranoside, (3beta, 6alpha, 16beta,24R) -20,24-epoxy-16,25-dihydroxy-3-(beta-D-xylopyranosyloxy) -9,19-cyclolanostan-6-yl;(3beta,6alpha,9beta,16beta,20R,24S)-16,25-dihydroxy-3-(beta-D-xylopyranosyloxy)-20,24-epoxy-9,19-cyclolanostan-6-yl beta-D-threo - hexopyranoside
Ifomula yemolekyuli:C41H68O14
Igama leMchiza:17- [5- (1-Hydroxyl-1-methyl-ethyl)- 2methyl-tetrahydro- furan-2-yl]-4,4,13,14-tetramethyl-tetradecahydro-cyclopropa[9,10]cyclopenta[a] phenanthren-16-ol-3-β-D-aracopyranosyl-6-β-D-glucoside
Mp:200 ~ 204℃
[α]D:-56.6(c,0.13 kwiDMF)
UV:λmax203 nm
ubunyulu:98%
Umthombo:imbotyi Astragalus membranaceus, Astragalus pubescens.
Ifomula yesakhiwo sekhemikhali ye-astragaloside IV
IiPropati zePhysicochemical
[imbonakalo]:umgubo omhlophe wekristale
[ubunyulu]:ngaphezulu kwe-98%, indlela yokufumanisa: HPLC
[umthombo wesityalo]:iingcambu zika Astragalus Alexandrinus Boiss, Astragalus dissectus, Astragalus membranaceus (Fisch.) Bungede ingcambu, Astragalus sieversianus Pall Ingcambu ye Astragalus spinosus Vahl, indawo yasemoyeni ye Astragalus spinosus Vahl.
[iipropati zemveliso]:Isicatshulwa se-Astragalus membranaceus ngumgubo omdaka omthubi.
[umiselo lomxholo]:misela yi-HPLC (Isihlomelo VI D, uMqulu I, i-Chinese Pharmacopoeia, uHlelo luka-2010).
Iimeko zeChromatographic kunye novavanyo lokusetyenziswa kwenkqubo} i-octadecyl silane bonded silica gel isetyenziswa njenge-filler, amanzi e-acetonitrile (32:68) asetyenziswa njengesigaba esihambayo, kwaye i-evaporative light scattering detector isetyenziselwa ukubonwa.Inani leepleyiti zethiyori aliyi kuba ngaphantsi kwe-4000 ngokwe-astragaloside IV peak.
Ukulungiswa kwesisombululo sereferensi, thatha inani elifanelekileyo lereferensi ye-astragaloside IV, ulinganise ngokuchanekileyo, kwaye ungeze i-methanol ukulungiselela isisombululo esiqulethe i-0.5mg nge-1ml.
Ukulungiswa kwesisombululo sovavanyo:thatha malunga ne-4G yomgubo kule mveliso, uyilinganise ngokuchanekileyo, uyibeke kwi-Soxhlet extractor, yongeza i-40ml ye-methanol, yifake ubusuku bonke, yongeza inani elifanelekileyo le-methanol, ukushisa kunye ne-reflux kwiiyure ze-4, buyisela i-solvent kwisicatshulwa kwaye ugxininise. ukuba yome, yongeza i-10ml yamanzi ukunyibilikisa intsalela, ishukumise kwaye uyikhuphe nge-n-butanol ehluthayo ngamaxesha e-4, 40ml ngexesha ngalinye, udibanise isisombululo se-n-butanol, kwaye uhlambe ngokupheleleyo ngesisombululo sovavanyo lwe-ammonia ngamaxesha e-2, 40ml nganye. ixesha, lahla isisombululo se-ammonia, ukhuphe isisombululo se-n-butanol, yongeza i-5ml yamanzi ukunyibilikisa intsalela, kwaye uyipholise, Ngokusebenzisa i-D101 macroporous adsorption column resin (ububanzi bangaphakathi: 37.5px, ukuphakama kwekholomu: 300px), elute nge-50ml yamanzi , lahla isisombululo samanzi, i-ethanol nge-30ml ye-40% ye-ethanol, lahla i-eluent, i-elute nge-80ml ye-70% ye-ethanol, iqokelele i-ethanol, ikhuphe i-eluent ibe yomile, nyibilikise intsalela nge-methanol, uyigqithisele kwi-flask ye-volumetric ye-5ml, yongeza methanol kwisikali, vuthulula kakuhle, kwayeuze uyifumane.
Indlela yokumisela:ukufunxa ngokuchanekileyo i-10% yesisombululo sereferensi ngokulandelelana μ l, 20 μ l.Isisombululo sovavanyo 20 nganye μ l.Yifake kwichromatograph yolwelo, yinqume, kwaye uyibale nge-logarithm equation yendlela yangaphandle yamanqaku amabini.
Ukubalwa njengemveliso eyomileyo, umxholo we-astragaloside IV (c41h68o14) awuyi kuba ngaphantsi kwe-0.040%
Isenzo se-Pharmacological
Awona macandelo asebenzayo e-Astragalus ziipolysaccharides kunye ne-astragaloside.I-Astragaloside yahlulwe yaba yi-astragaloside I, i-astragaloside II kunye ne-astragaloside IV.Phakathi kwazo, i-astragaloside IV, i-astragaloside IV, inowona msebenzi ubalaseleyo webhayoloji.I-Astragaloside IV ayinayo kuphela impembelelo ye-Astragalus polysaccharides, kodwa kunye nemiphumo engenakuqhathaniswa ye-Astragalus polysaccharides.Ukuqina kwayo okusebenzayo kungaphezulu kokuphindwe kabini kweyesiqhelo i-astragalus polysaccharides, kwaye isiphumo sayo sokulwa nentsholongwane sinamaxesha angama-30 e-Astragalus polysaccharides.Ngenxa yomxholo ophantsi kunye nesiphumo esihle, ikwabizwa ngokuba yi "super astragalus polysaccharide".
1.Ukwandisa ukhuseleko kunye nokuxhathisa izifo.
Inokukhuphela ngaphandle ngokuthe ngqo nangokungakhethi imizimba yangaphandle ehlasela umzimba, ikhuthaze ukhuselo lomzimba olukhethekileyo, lokhuselo lomzimba kunye nokungachanekanga, kwaye iphucule ukuxhathisa kwezifo emzimbeni.Inokukhuthaza ukuveliswa kwe-antibody, kwaye ikhulise kakhulu inani le-antibody eyenza iiseli kunye nexabiso lovavanyo lwe-hemolysis.I-Astragaloside IV inokuphucula ngokubonakalayo inqanaba lenguqu ye-lymphocyte kunye nesantya sokwakheka kwe-E-rosette yenkukhu ezigonywe yicoccidia.It is a activator olusebenzayo inkqubo monocyte macrophage.I-Astragaloside IV inokuphucula i-oxidation, i-GSH-Px kunye nemisebenzi ye-SOD kumalungu omzimba omzimba, kunye nokuphucula ukhuseleko lomzimba kunye nemisebenzi yokubeka iliso kwi-immune.
2.Impembelelo yeAntiviral.
Umgaqo wayo we-antiviral: ukuvuselela umsebenzi we-macrophages kunye neeseli ze-T, ukwandisa inani le-E-ring ukwenza iiseli, ukunyanzeliswa kwe-cytokines, ukukhuthaza ukuqaliswa kwe-interleukin, kwaye wenze umzimba wezilwanyana uvelise i-interferon engapheliyo, ukuze ufezekise injongo ye-antiviral.Iziphumo zibonise ukuba izinga lokukhusela elipheleleyo le-astragaloside IV kwi-IBD yayingu-98.33%, enokuthintela ngokufanelekileyo kwaye iphathe i-IBD, kwaye kwakungekho mmahluko obalulekileyo xa kuthelekiswa nesisombululo esiphezulu se-yolk yeqanda.I-Astragaloside inokuphucula umsebenzi we-antioxidant enzymes emzimbeni, ukunciphisa umxholo we-LP0, ukunciphisa umonakalo weentlobo ze-oksijini esebenzayo, kwaye ngaloo ndlela inciphisa izinga lokufa kunye nokufa kwe-MD.Inokuphucula umsebenzi ophantsi wokuzivikela obangelwa yi-tumor, ikhuthaze ukusebenza kweeseli zokuzivikela, ikhulule izinto ezingapheliyo, kwaye ithintele ukubulawa kunye nokuvinjelwa kweeseli ze-tumor ezibangelwa yi-peroxidation;I-Astragaloside a inokuthintela ukukhula kwentsholongwane yomkhuhlane kunye nomsebenzi we-sialidase.Inefuthe elibalulekileyo kumsebenzi we-influenza virus cell membrane kunye ne-adsorption kunye nokungena kwentsholongwane kwiiseli ezibuthathaka.Izinga lokufa kunye neqanda lokubeka iqanda lenkukhu liye lancitshiswa kakhulu, kwaye izinga lokubeka iqanda kunye nokubuyiswa kwekhwalithi yeqanda kwakungcono kakhulu kuneqela le-amantadine kuphela lokulawula, kwaye umphumo we-Astragalus polysaccharide wawungabonakali;I-Astragaloside IV inokubulala okunamandla kunye neziphumo ezithintelayo kwintsholongwane.Isiseko kukuba ukusetyenziswa kwe-astragaloside IV kuphambi kokufunyanwa kokusuleleka kwintsholongwane ye-Nd, ngoko ke kungcono ukusebenzisa i-astragaloside IV ixesha elide, i-Avian myeloblastic leukemia (AMB) i-3 yeentsuku ze-AA ze-broilers zondla i-astragaloside IV kunye nosulelo. yentsholongwane ye-AMB, inokunciphisa izinga lokufa kunye nokufa kwe-AMB, ukwandisa umxholo we-LPO kwizitho zokuzivikela ezifana ne-spleen kunye ne-thymus, iphucula kakhulu umphumo wokukhupha we-spleen kunye ne-thymus kunye namanye amalungu omzimba kwi-myeloid ephuma kwiiseli ze-tumor.Okwesibini, i-astragaloside IV ineziphumo ezicacileyo zothintelo kunye nonyango kwizifo zokuphefumla ezifana ne-laryngotracheitis esosulelayo.Sebenzisa.
3. Isiphumo sokulwa noxinzelelo.
I-Astragaloside IV inokuthintela i-adrenal hyperplasia kunye ne-thymus atrophy ngexesha lesilumkiso sempendulo yoxinzelelo, kwaye ithintele utshintsho olungaqhelekanga kwixesha lokumelana kunye nexesha lokungaphumeleli kwempendulo yoxinzelelo, ukuze udlale indima yokulwa noxinzelelo, ngakumbi unomgaqo obalulekileyo weendlela ezimbini. impembelelo kwi-enzymes kwinkqubo ye-metabolism yezondlo, kwaye inciphisa kwaye isuse impembelelo yoxinzelelo lobushushu kumsebenzi we-physiological womzimba ukuya kwinqanaba elithile.
4. Njengomkhuthazi wokukhula.
Inokuphucula i-physiological metabolism yeeseli, ikhuthaze ukujikeleza kwegazi, iphucule imetabolism yomzimba wezilwanyana, kwaye idlale indima yesondlo kunye nokhathalelo lwempilo.Uphando lubonisa ukuba lunokukhuthaza ukukhula kwe-bifidobacteria kunye ne-lactic acid bacteria kwaye inefuthe le-probiotics.
5. I-Astragaloside IV inokuphucula umsebenzi we-cardiopulmonary.
Yomeleza isivumelwano sentliziyo, khusela i-myocardium kwaye uxhathise ukungaphumeleli kwentliziyo.Kwakhona kunemiphumo yokukhusela isibindi, i-anti-inflammatory kunye ne-analgesic.Ingasetyenziswa njengonyango lwe-adjuvant kwizifo ezahlukeneyo zentsholongwane kunye neebhaktheriya.
