Iimveliso

Inkcazo:I-Danshensu iyinxalenye esebenzayo ye-Salvia miltiorrhiza, enokuthi isebenze i-Nrf2 indlela yokubonisa kunye nokukhusela inkqubo ye-cardiovascular system.
Iindidi eziyeleleneyo: indlela yomqondiso > > autophagy > > autophagy
Indlela yomqondiso > > NF- κ B indlela yomqondiso > > keap1-nrf2
Indawo yophando > > isifo sentliziyo
Iimveliso zendalo > > iiasidi ze-benzoic

Isifundo se-In Vitro:I-Danshensu (i-DSS) yanciphisa kakhulu amanqanaba e-marker enzymes (i-creatine kinase kunye ne-lactate dehydrogenase) ye-coronary outflow kunye ne-myocardial infarction size.Oku kunokukhuthaza kakhulu ukubuyiswa komsebenzi wentliziyo emva kokulimala kwe-I / R.I-DSS nayo inomsebenzi wokutshabalalisa i-ROS kwaye ikhuthaza umsebenzi we-antioxidants ongapheliyo njenge-SOD, ikati, i-MDA, i-GSH-Px kunye ne-HO-1 ngokwenza i-nuclear factor erythrocyte-2 enxulumene ne-2 (Nrf2) indlela yokubonisa i-Akt kunye ne-ERK1.I-2 kuhlalutyo lwe-blot yaseNtshona [2].

KwiSifundo seVivo:unyango olunzima kunye nedosi enye yeDanshensu ayizange itshintshe i-plasma tHcy kwiigundane ezine-tHcy eqhelekileyo.Ngokwahlukileyo, iDanshensu yanciphisa kakhulu i-tHcy kwiimpuku ezine-tHcy ephakamileyo.Amanqanaba aphezulu e-cysteine ​​kunye ne-glutathione emva konyango kunye ne-Danshensu ibonisa ukuba umphumo wayo wokunciphisa i-tHcy kukunyusa umsebenzi we-trans vulcanization pathway [1].

Imifuniselo yezilwanyana:zonke iikhemikhali zachithwa kwi-saline ngaphandle kwe-tolcapone echithwe kwi-saline equkethe i-20% (V / V) isikhonkwane se-200. Ngethuba lovavanyo, iigundane zazila ukudla ngobusuku kwaye zabelwa ngokungaqhelekanga kumaqela ahlukeneyo.Emva kwe-anesthesia ye-ether, malunga ne-200 yasuswa kwi-orbital sinus μ L igazi, ngoko ngokukhawuleza i-disinfected nge-alcohol kwaye ucinezele ngekotoni.Iisampulu zegazi zaqokelelwa ngokukhawuleza kwiibhubhu ze-polypropylene ezine-heparin sodium kunye ne-centrifuged kwi-5000 g kwi-5 ° C kwimizuzu ye-3.Iisampuli zeplasma ezilungisiweyo zagcinwa ku-20 ℃ kwaye zahlalutywa kwiiyure ezingama-48.

IiReferensi:[1] YG Cao, et al.Iziphumo eziluncedo zedanshensu, icandelo elisebenzayo leSalvia miltiorrhiza, kwi-homocysteine ​​metabolism ngendlela ye-trans-sulphuration kwiigundane.Br J Pharmacol.2009 Jun;157(3): 482–490.

[2].U-Yu J, kunye nabanye.I-Danshensu ikhusela intliziyo esecaleni kwi-ischemia yokulimala kwakhona ngokusebenza kwe-Akt / ERK1 / 2 / Nrf2 yokubonisa.Int J Clin Exp Med.2015 Sep 15;8(9):14793-804.