Iimveliso

Ekujoliswe kuko:JNK1 jnk2 NF- κ B

Isifundo se-in vitro:I-isovitexin ikhusela i-LPS eyenza umonakalo we-oxidative ngokuvimbela ukuveliswa kwe-ROS ye-intracellular, kwaye yenza buthathaka umphumo we-H2O2 ekusebenzeni kweeseli.Iqulethe i-LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) yayingenayo i-cytotoxicity kwiiseli ezikrwada ezingama-264.7, kodwa i-200 μ G / ml isovitexin ibonise i-cytotoxicity ebalulekileyo.I-Isovitexin (25,50) μ G / ml) inqanda i-LPS eyenziwe i-TNF- α, Ukunyuka kwamanqanaba e-IL-6, iNOS kunye ne-COX-2.I-Isovitexin (25,50) μ G / ml) iphinde ivimbele i-I kwiiseli eziluhlaza ze-264.7 κ B α Phosphorylation kunye nokuthotywa, okuhambelana nomphumo we-JNK1 / 2 inhibitor [1].

Izifundo ze-vivo:I-isovitexin (i-50 kunye ne-100 mg / kg, i-IP) ibangele utshintsho oluncinci lwe-histopathological kumacandelo emiphunga kwaye yanciphisa inani leeseli ezivuthayo kwiigundane ezenziwe yi-LPS.I-Heteroeosinophils (i-50 kunye ne-100 mg / kg, i-IP) iyancipha i-TNF nge- α Kwaye ukuveliswa kwe-IL-6, ukuveliswa kwe-ROS, i-MPO kunye nomxholo we-MDA, ukunyuka kwe-SOD kunye ne-GSH, kunye nokuthintela i-LPS ibangele ukuvuvukala kunye noxinzelelo lwe-oxidative kwi-LPS eyenza u-Ali iigundane.Kwaye inqanda ngokufanelekileyo iprotheni ye-iNOS kunye ne-COX-2 [1].I-Isovitexin (25,50, 100 mg / kg) yehlisa izinga lokusinda kwe-LPS / D-gal eyenza ukulimala kwesibindi kwiimpuku ngendlela exhomekeke kwidosi.I-Isovitexin iphinde ithintele i-NF- κ B kwaye phezulu ilawula i-LPS / i-D-gal eyenziwe nge-Nrf2 kunye ne-HO-1 kwiigundane [2].

Umfuniselo weseli:ukusebenza kweseli kwamiselwa luvavanyo lwe-MTT.Iiseli ze-264.7 ezikrwada zifakwe kwiiplati ze-96 kakuhle (1) × iiseli ze-104 / kakuhle) kunye neentlobo ezahlukeneyo ze-isovitexin (i-concentration yokugqibela: 0-200) μ G / ml) kunye ne-LPS (2 μ G / ml) kwiiyure ezingama-24.Ukongeza, sebenzisa IV (25 okanye 50 μ G / ml) pretreated iiseli 1 iyure, emva koko H 2O 2 (300) yongezwa μ M）。 Emva kweeyure ezingama-24, MTT (5 mg / ml) yongezwa iiseli ze emva koko zifakwe iiyure ezi-4 [1].

Umfuniselo wezilwanyana:iimpuku [1] ukwenzela ukuseka Ali imodeli, iimpuku zahlulwe ngokungenamkhethe kumaqela 6: ulawulo (saline), kuphela isovitexin (100 mg / kg, inyibilikiswe 0.5% DMSO), kuphela LPS (0.5 mg / kg, inyibilikiswe saline ), LPS (0.5 mg / kg) + isovitexin (50 okanye 100 mg / kg) kunye ne-LPS (0.5 mg / kg) + i-dexamethasone (DEX, 5 mg / kg, echithwe kwi-saline).I-Isovitexin okanye i-DEX (5 mg / kg) i-isovitexin yalawulwa.Emva kokuvezwa kwi-isovitexin okanye i-DEX yeyure ye-1, iigundane zaye zahlanjululwa nge-ether kwaye i-LPS yayilawulwa nge-intranasally (in) ukwenza ukulimala kwemiphunga.Izilwanyana zaxhatshazwa kwiiyure ze-12 emva kokulawulwa kwe-LPS.Ngoko ke, i-bronchoalveolar lavage fluid (BALF) kunye neesampuli zezicubu zemiphunga zavunwa ukulinganisa amanqanaba e-cytokine;Isizukulwana seROS;Imisebenzi ye-SOD, GSH, MDA kunye ne-MPO;Kwaye ukubonakaliswa kwe-COX-2, iNOS, i-HO-1 kunye neeprotheni ze-Nrf2 [1].