Jujuboside A CAS No. 55466-04-1
Ulwazi oluBalulekileyo
Isiteketiso sesiTshayina:ijujube kernel saponin A, jujube kernel saponin A;Ijube kernel saponin A (esemgangathweni)
Igama lesiNgesi:Jujuboside A
Umthombo wesityalo:iphuma kwimbewu ye jujube yasendle, ekwaziwa njenge jujube kernel kunye ne wild jujube core
Ifomula yemolekyuli:C58H94O26
Ubunzima bemolekyuli:1207.35
Inombolo yeCAS.55466-04-1
Ukusebenza kwe-Bioactivity yeJujuboside A
Ekujoliswe kuko:I-GABA receptor, mTOR, PI3K, Akt [1] [2]
Isifundo se-in vitro:idosi ephantsi 41 μ Jujuboside A ye-M (malunga ne-0.05 g / L) yenza i-GABA (a) i-receptors ngexesha le-24 kunye ne-72 unyango lwe-h α 1, α 5, β 2 subunit mRNA yanda kakhulu.I-dose ephezulu 82 μ M (malunga ne-0.1 g / L) I-Jujuboside A yanda kakhulu i-GABA (a) i-receptors kwi-24 h α 1, α 5 i-subunit mRNA amanqanaba kunye nokunciphisa i-β 2 i-subunit ye-mRNA kunye nokunciphisa i-GABA (a) i-subunit ye-receptor α 1, Inkcazo ye-β 2 mRNA yaphathwa kwi-72 h [1].I-Jujuboside Unyango lwangaphambili lunokubuyisela umva ukwehla kokusebenza kweeseli kwaye yenze ngcono i-ISO eyenzelwe ukwenzakala kweseli ye-H9c2.I-Jujuboside A inokukhawulezisa i-phosphorylation ye-PI3K, i-Akt kunye ne-mTOR.IJujuboside A inokunciphisa kakhulu ubungakanani beprotheyini ehambelana ne-microtubule LC3-II / I kwiiseli ze-H9c2 [2]
Isifundo se-vivo:emini (9: 00-15: 00), i-jujubosides yandisa kakhulu ubuthongo obupheleleyo kunye nokunyakaza kwamehlo okukhawuleza (REM) ukulala, kodwa kwakungekho nefuthe elibalulekileyo kwi-non-REM (NREM) yokulala.Ebusuku (21:00-3:00), i-jujubosides yandisa kakhulu ubuthongo obupheleleyo kunye nokulala kwe-NREM, ngakumbi ukulala okuncinci, kodwa kwakungekho mpembelelo ebalulekileyo kubuthongo be-REM kunye nokulala okucothayo (SWS) [3].Unyango lwe-Intraventricular kunye ne-jjuboside a yancitshiswa kakhulu nge-Y-maze, ukuphepha okusebenzayo kunye nemilinganiselo ye-Morris yamanzi imilinganiselo ye-maze β 1-42 ekhuthaza ukufunda kunye nokukhubazeka kwememori kwiigundane.I-injection ye-Intracerebroventricular ye-Jujuboside A i-hippocampal iyancipha amanqanaba e-β 1-42 athintele kakhulu imisebenzi ye-acetylcholinesterase (AChE) kwaye akukho, kwaye yanciphisa umxholo owandisiweyo we-malondialdehyde (MDA) kwi-hippocampus kunye ne-cerebral cortex yeegundane ezifakwe kwi-lateral β1. [4].
Umfuniselo weseli:emva kweentsuku ze-7 zenkcubeko kwi-vitro, iiseli zaye zavezwa ngokuqhubekayo kwiiyure ze-24 okanye iiyure ze-72, kwaye azizange zivelele kwi-medium equkethe i-Jujuboside A okanye i-diazepam okanye zombini.IVector yongezwa kwiqela lolawulo;Iqela le-Diazepam longezwe i-10 μ M diazepam;I-Jujuboside A82 μ M (malunga ne-0.1g / L) kunye ne-41 μ M (malunga ne-0.05 g / L) yongezwa ku-jua-h (idosi ephezulu i-Jujuboside A) kunye ne-jua-l (idosi ephantsi Jujuboside A) amaqela, ngokulandelelana.Iyonke i-RNA yahlukaniswa kwiiseli ukuze kuhlalutywe ngakumbi [1].
Umfuniselo wezilwanyana:iimpuku: a yatofwa nge-ICV β 1-42 eyenzelwe ukonakala kwengqondo kwiimpuku.Emva koko, iigundane zafakwa nge-Jujuboside A (0.02 kunye ne-0.2 mg / kg) intraventricular (ICV) kwiintsuku ze-5.I-Y maze, ukuphepha okusebenzayo kunye novavanyo lwe-maze yamanzi kaMorris lwenziwa kwiimpuku [1].
Igama lesiNgesi likaJujuboside A
α-L-arabinopyranoside, (3β,16β,23R)-16,23:16,30-diepoxy-20-hydroxydammar-24-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1- >2)-O-[O-β-D-glucopyranosyl-(1->6)-O-[β-D-xylopyranosyl-(1->2)]-β-D-glucopyranosyl-(1->3 )]-
(3β,16β,23R)-20-Hydroxy-16,23:16,30-diepoxydammar-24-en-3-yl 6-deoxy-α-L-mannopyranosyl-(1->2)-[β-D -glucopyranosyl-(1->6)-[β-D-xylopyranosyl-(1->2)]-β-D-glucopyranosyl-(1->3)]-α -L-arabinopyranoside
jujuboside C
α-L-Arabinopyranoside, (3β,16β,23R)-16,23:16,30-diepoxy-20-hydroxydammar-24-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1- >2)-O-[O-β-D-glucopyranosyl-(1->6)-O-[β-D-xylopyranosyl-(1->2)]- β-D-glucopyranosyl-(1->3 )]-
JujubosideA
(3β,16β,23R)-20-Hydroxy-16,23:16,30-diepoxydammar-24-en-3-yl 6-deoxy-α-L-mannopyranosyl-(1->2)-[β-D -glucopyranosyl-(1->6)-[β-D-xylopyranosyl-(1->2)]-β-D-glucopyranosyl-(1->3)]-α-L-arabinopyranoside
