Iimveliso

Inkcazo:
pachymic acid yi triterpenoid evela P. cocos.I-Pachymic acid inqanda iindlela zokubonisa i-Akt kunye ne-ERK.

Iindidi ezinxulumeneyo:
icandelo lophando > > umhlaza
Iimveliso zendalo > > i-terpenoids kunye ne-glycosides

Ekujoliswe kuko:
Akt
ERK

Isifundo se-In Vitro:
i-pachymic acid (PA) inokuthintela i-tumorigenesis yomhlaza we-gallbladder ebandakanya i-Akt kunye ne-ERK iindlela zokubonisa.Unyango lwe-Pachymic acid (PA) luthintele kakhulu iindlela ze-Rho a, i-Akt kunye ne-ERK kwiiseli zomhlaza we-gallbladder.Unyango lwe-Pachymic acid (PA) lunokunciphisa i-PCNA, i-ICAM-1, i-RhoA, i-p-Akt kunye ne-perk ngendlela exhomekeke kumthamo.Iiyure ezili-12 emva konyango, i-10 μ G / ml i-Poria acid (PA) inqanda ukukhula kweeseli kwi-concentration ye-30 μ G / ml iyancipha ngakumbi ukukhula kweeseli.Ukukhula kweeseli kuye kwathintelwa ngexesha - kunye nendlela exhomekeke kwithamo.Emva kweeyure ezingama-48 zonyango, ugxininiso lwaluyi-10 μ g / mL, 20 μ G / ml kunye ne-30 μ Pachymic acid (PA) kwi-g / ml inhibited ukukhula kweeseli malunga ne-25%, 40% kunye ne-70%.I-Pachymic acid (PA) iphinda inqanda ukukhula kweeseli zomhlaza we-gallbladder ngendlela exhomekeke kwixesha kunye ne-dose-dependent [1].

KwiSifundo seVivo:
ukuze kuhlolwe umsebenzi we-antitumor we-pachymic acid (PA) kwi-vivo, umhlaza wemiphunga yomntu we-nci-h23 imodeli ye-tumor xenograft yasetyenziswa.I-Pachymic acid (PA) ithintele kakhulu ukukhula kwethumba kwiidosi ze-30 kunye ne-60 mg / kg iintsuku ezingama-21 xa kuthelekiswa neqela lolawulo [2].

Uvavanyo lweSeli:
Ikiti yokubala iseli-8 (CCK-8) yayisetyenziselwa ukuvavanya isiphumo esichasene nokwanda kwe-pachymic acid (PA) kwiiseli ze-GBC-SD.Ngamafutshane, emva konyango oluchaziweyo, yongeza i-10 kumngxuma ngamnye μ Lcck-8 isisombululo, kwaye emva kokufukanyelwa enye, ukufunxa kulinganiswe kwi-450 nm usebenzisa umfundi we-microplate [1].

Uvavanyo lwezilwanyana:
iimpuku [2] wasebenzisa iimpuku ezibhinqileyo athymic nude ubudala 4-5 iiveki.Iiseli ze-nci-h23 ezikhule ngokwamandla (ku-100 μ 5 kwii-LPBs × 106) zitofwe ngaphantsi kwesikhumba kwiflanki yasekunene yempuku nganye.I-tumor xenografts yakhuliswa ibe yi-avareji yobukhulu be-100-200mm 3 kwaye yabelwa ngokungaqhelekanga kumaqela amane onyango ahlukeneyo (iigundane ezi-6 kwiqela ngalinye): (a) ulawulo lwezithuthi (0.1% DMSO kwi-saline eqhelekileyo);(b) Pachymic acid (PA) 10 mg / kg;(c) PA 30 mg / kg;(Dd) PA 60mg / kg. Iimpuku zanikwa pa nge-intraperitoneal (IP) inaliti kwiiveki ezi-3 (iintsuku ezi-5 / ngeveki).Ubungakanani be-tumor bulinganiswa kwii-axes ezimbini ngoncedo lwe-vernier caliper, kwaye umthamo we-tumor (mm3) ubalwa.

Isalathiso:
[1].UChen Y, et al.I-Pachymic acid inqanda i-tumorigenesis kwiiseli ze-gallbladder carcinoma.Int J Clin Exp Med.2015 Oktobha 15; 8(10): 17781-8.
[2].UMa J, et al.I-Pachymic acid ibangela i-apoptosis ngokuvula i-ROS exhomekeke kwi-JNK kunye ne-ER iindlela zoxinzelelo kwiiseli zomhlaza wemiphunga.Cancer Cell Int.2015 Agasti 5;15:78.