I-Paeoniflorin CAS No. 23180-57-6
Ulwazi oluBalulekileyo
Ikwaziwa ngokuba yi-paeoniflorin, yipinane monoterpene ekrakra glycoside ebekwe yodwa kwi-peony ebomvu kunye ne-peony emhlophe.Ingumgubo we-amorphous we-hygroscopic.Ikhona kwiingcambu zePaeonia, peony, peony emfusa kunye nezinye izityalo zeRanunculaceae.Ubuthi bale crystal buphantsi kakhulu.
[igama lekhemikhali]5beta-[(Benzoyloxy)methyl]tetrahydro-5-hydroxy-2-methyl-2,5-methano-1H-3,4-dioxacyclobuta[cd]pentalen-1alpha(2H)-yl-beta-D-glucopyranoside
[ifomula yemolekyuli]C23H28O11
【CASHayi】23180-57-6
Ubunyulu: ngaphezu kwe-98%, indlela yokufumanisa: HPLC.
[umthombo]iingcambu zePaeonia albiflora pall, P. suffrsticosa Andr, P. delarayi Franch, isityalo saseRanunculaceae, umxholo we-radix paeoniae Rubr ngowona uphezulu.
[Inkcazo]10%, 20%, 30%, 50%, 90%, 98%
[AesebenzayoIisithako ] Iyonke iglucosides ye-Paeonia (TGP) ligama jikelele le-paeoniflorin, i-hydroxy paeoniflorin, i-paeoniflorin, i-albiflorin kunye ne-benzoyl paeoniflorin, ebizwa ngokuba yi-TGP ngokufutshane.
Iimpawu zomzimba kunye neekhemikhali
I-hygroscopic amorphous Tan powder (90% ivaliwe ngumgubo omhlophe)[ α] 16D-12.8。 (C = 4.6, i-methanol), i-tetraacetate i-crystal acicular engenambala, indawo yokunyibilika: 196 ℃.I-Paeoniflorin izinzile kwindawo ene-acidic (pH 2 ~ 6) kwaye ayizinzile kwindawo ye-alkaline.
Ukumiselwa komxholo
Ngokubanzi, indlela yoku-1 kunye nendlela yesi-2 nayo ingasetyenziselwa ukukhangela.Indlela ye-1 isetyenziswe ngokufanelekileyo kwimveliso yomxholo ophezulu, enokunceda abasebenzi ukuba bagwebe ngcono ukucoceka kweemveliso.I-substance yereferensi kulula ukubola emva kokuchithwa.
1.Kwamiselwa yichromatography yolwelo olusebenza kakhulu (Isihlomelo VI d).Iimeko zechromatographic kunye nokufaneleka kwenkqubo zavavanywa kunye ne-Octadecyl silane eboshiweyo ye-silica gel njenge-filler;I-Acetonitrile-0.1% isisombululo se-phosphoric acid (14: 86) sisetyenziswe njengesigaba seselula;Ubude bokubona ubude yi-230nm.Inani leepleyiti zethiyori ezibalwe ngokwe-paeoniflorin peak aziyi kuba ngaphantsi kwe-2000. Ukulungiswa kwesisombululo sereferensi: ukulinganisa ngokuchanekileyo isixa esifanelekileyo sesisombululo sereferensi ye-paeoniflorin kwaye ungeze i-methanol ukulungiselela i-60% ye-paeoniflorin nge-1ml μ G isisombululo.
2.Ukuphucula indlela yokuzimisela ye-paeoniflorin kwi-Radix Paeoniae Alba.Iindlela: iindlela ze-China Pharmacopoeia kunye neendlela eziphuculweyo zathelekiswa.Isigaba esihambayo ibingamanzi e-methanol (30:70) kwaye ubude bokubona ubuyi-230nm.isiphumoUbudlelwane bomgca wale ndlela buhle (r = 0.9995).Umyinge wokubuyisela yi-101.518% kunye ne-RSD yi-1.682%.Isiphelo: indlela ephuculweyo ilula kwaye ichanekile, enokunciphisa ubuthi be-solvents yezinto eziphilayo kubantu kunye nongcoliseko lwendalo, kwaye inike isiseko sokubhekisela ekumiselweni kwe-paeoniflorin ekusebenzeni.
Indlela yokuzimisela
Ukumiselwa kwe-paeoniflorin yi-HPLC
Ubungakanani besicelo:le ndlela isebenzisa HPLC ukumisela umxholo paeoniflorin kwiipilisi Guizhi Fuling.
Indlela ifanelekile kwipilisi ye-Guizhi Fuling.
Indlela yomgaqo:faka isampuli yovavanyo kwi-flask ye-conical, yongeza inani elifanelekileyo le-ethanol edibeneyo yokutsalwa kwe-ultrasonic, yipholise, ishukumise kakuhle, ihluze, i-filtrate ingena kwi-chromatograph yolwelo oluphezulu lwe-chromatographic yokwahlukana kwe-chromatographic, sebenzisa i-ultraviolet absorption detector ukubona ixabiso lokufunxa le-paeoniflorin kubude be-wavelength ye-230nm, kwaye ubale umxholo wayo.
