Iimveliso

Inkcazo:
I-prim-o-glucosylcimifugin ineempembelelo ezinamandla zokuchasa ukudumba.Ukubonakaliswa kwe-iNOS kunye ne-COX-2 kunokuvinjelwa ngokulawula i-JAK2 / STAT3 isignali yokudluliselwa

Iindidi ezifanelekileyo:
Indlela yokubonakalisa imiqondiso > > ukukhuseleka kunye nokuvuvukala > > i-nitric oxide synthase
Inkalo yophando > > ukudumba / ukunganyangeki
Iimveliso zendalo > > abanye

Ekujoliswe kuko:INOS
COX-2

Isifundo se-In Vitro:
I-prim-o-glucosylglycine (POG) ngowona mxholo uphezulu we-chromone kunye nenye yezinto eziphambili ezisebenzayo kwi-Fangfeng (RS).I-Prim-o-glucosylglycine idlala indima echasene nokuvuvukala kwi-macrophages eluhlaza ye-264.7 ngokuvimbela ukuhanjiswa kwesignali ye-JAK2 / STAT3 kunye nokuthintela ukubonakaliswa kwe-iNOS kunye ne-COX-2.I-cytotoxicity ye-prim-o-glucoside kwi-LPS evuliwe ekrwada ye-264.7 macrophages yalinganiswa.Sebenzisa i-LPS (i-1 μ G / ml) kunye nokwanda koxinzelelo lwe-prim-o-glucosylglycine (15, 50 kunye ne-100 μ G / ml) kwiiyure ezingama-24, kunye nokusebenza kweeseli kwavavanywa ngovavanyo lweCCK-8.Xa kuthelekiswa neeseli eziphathwayo ze-DMSO (ulawulo), iiyure ezingama-24 kunye nokuvezwa kwi-15-100 μ Emva kwe-g / ml prim-o-glucoside, ukusebenza kweseli akuzange kuchaphazeleke kakhulu.Ukuze ufunde i-anti-inflammatory effect ye-prim-o-glucosylglycine, nokuba i-prim-o-glucosylglycine inokuchaphazela i-NO synthesis yavavanywa kwi-LPS evuliwe ekrwada iiseli ezingama-264.7.Sebenzisa i-LPS (1 μ G / ml) kunye neentlobo ezahlukeneyo ze-prim-o-glucosylglycine (15, 50 kunye ne-100 μ G / ml) kwiiyure ezingama-24.Ugxininiso aluzange lulinganiswe kwinkcubeko ephezulu ngokuphendula kukaGriess.Uxinaniso lwe-no kwinkcubeko ephezulu lunyuke kakhulu ngokuvezwa kwe-LPS, kwaye i-prim-o-glucoside ithintele kakhulu i-LPS yenze ukuba kungabikho mveliso ngendlela exhomekeke kugxininiso [1].
KwiSifundo seVivo
I-bronchoalveolar lavage fluid (BALF) yaqokelelwa kwiiyure ze-7 emva kokulawulwa kwe-lipopolysaccharide (LPS), kunye nenqanaba le-cytokine kwi-BALF yalinganiswa ngu-ELISA.Xa kuthelekiswa neqela lolawulo, i-TNF kwi-BALF- α, IL-1 β Kwaye amanqanaba e-IL-6 anyuke kakhulu.Nangona kunjalo, ukunyangwa kwangaphambili nge-prime-o-glucosylglycine (2.5, 5 okanye 10 mg / kg) iphantsi kakhulu elawulwa yi-TNF- α, IL-1 β kunye ne-IL-6 ngendlela exhomekeke kwidosi (P <0.05, P <0.05, 0.01) ) [1].

Uvavanyo lweSeli
I-cell counting Kit (CCK-8) yayisetyenziselwa ukumisela i-cytotoxic concentration ye-prim-o-glucosylglycine.Ngamafutshane, iiseli eziluhlaza ze-264.7 zaphathwa nge-1 ngequla ngalinye × Ubuninzi beeseli ze-104 zahlanjululwa kumaqula angama-96 kwaye zifakwe ubusuku bonke.Emva koko sebenzisa i-1 μ Iiseli zavuselelwa nge-g / ml ye-LPS kwaye ziphathwe ngokugxininiswa okuhlukeneyo kwe-prim-o-glucosylglycine (15, 50 kunye ne-100 μ g / mL; iMedchem express, Princeton, NJ, USA) okanye unyango lwe-DMSO.Emva kokufukanyelwa kwi-37 ℃ iiyure ezingama-24, isisombululo seCCK-8 songezwa kwiqula ngalinye kwaye sifakelwe enye iyure.I-Absorbance ilinganiswa kwi-450 nm usebenzisa i-microplate reader [1].

Animal Experiment
iimpuku [1] BALB / C inkunzi iimpuku, 8 iiveki ubudala, ubunzima malunga 18 ukuba 20g.Iigundane zahlulwa ngokungenamkhethe zibe ngamaqela e-5: iqela lokulawula;Iqela le-LPS;LPS + prime-o-glucosylglycine (2.5, 5 okanye 10mg / kg ubunzima bomzimba).I-Prime-o-glucosylglycine ilawulwa nge-intraperitoneally.Emva kweyure eyi-1, iimpuku kwiqela le-LPS kunye ne-LPS + iqela le-prime-o-glucosylglycine lanikwa i-50 mg / L intranasal (in) (200 mg / L) μ LLPs ukubangela ukwenzakala okukhulu kwemiphunga.Iigundane zokulawula zanikwa i-50% ye-intranasal (in) ngaphandle kwe-LPS μ LPBS
[1]

Isalathiso:
[1].UZhou J, kunye nabanye.I-Prim-O-glucosylcimifugin Iyanciphisa i-Lipopolysaccharide i-Inflammatory Response kwi-RAW 264.7 Macrophages.Pharmacogn Mag.2017 Jul-Sep;13(51):378-384.
[2].UChen N, kunye nabanye.I-Prime-O-glucosylcimifugin ithoba i-lipopolysaccharide-induced acute lung injury kwiimpuku.I-Immunopharmacol.2013 Jun;16(2):139-47.