Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
Ulwazi oluBalulekileyo
I-Salvianolic acid B kukuxinaniswa kweemolekyuli ezintathu zeDanshensu kunye nemolekyuli enye ye-caffeic acid.Yenye yeeasidi ze-salvianolic ezifundwe ngakumbi.Ineziphumo ezibalulekileyo ze-pharmacological kwintliziyo, ingqondo, isibindi, izintso kunye namanye amalungu.Le mveliso ineempembelelo zokukhuthaza ukujikeleza kwegazi kunye nokususa i-stasis yegazi, i-dredging meridians kunye ne-activation collaterals.Isetyenziselwa ikakhulu ukunyanga i-ischemic stroke ebangelwa yi-blood stasis evalela i-meridians, efana nokuba ndindisholo kwesiqingatha somzimba kunye nemilenze, ubuthathaka, iintlungu zekhontrakthi, ukusilela kwemoto, ukuphambuka komlomo kunye namehlo, njl.
Isifaniso:i-salvianolic acid B, i-salvianolic acid B, i-salvianolic acid B
Igama lesiNgesi:i-salvianolic acid B
Ifomula yemolekyuli:c36h30o16
Ubunzima bemolekyuli:718.62
Inombolo yeCAS:115939-25-8
Indlela yokubona:HPLC ≥ 98%
Iinkcukacha:10mg, 20mg, 100mg, 500mg, 1g (inokupakishwa ngokweemfuno zabathengi)
Umsebenzi kunye nokusetyenziswa:Le mveliso isetyenziselwa ukumisela umxholo.
Iimpawu zomzimba kunye neekhemikhali
Iinkcazelo ngeempawu:imveliso quasi umgubo omhlophe.
Incasa iyakrakra kwaye i-astringent, kunye nepropathi ebangela ukufuma.Inyibilika emanzini, ethanol kunye nemethanol.
I-Salvianolic acid B yenziwe ngokuxinwa kwee-molecule ze-3 ze-salvianolic acid kunye ne-molecule ye-caffeic acid.Inamaqela amabini e-carboxyl kwaye ikhona ngendlela yeetyuwa ezahlukeneyo (K +, Ca2 +, Na +, NH4 +, njl.).Kwinkqubo ye-Decoction kunye nokugxila, inxalenye encinci ye-salvianolic acid B i-hydrolyzed kwi-oxalic acid emfusa kunye ne-salvianolic acid, kunye nenxalenye ye-salvianolic acid B iba yi-rosmarinic acid phantsi kweemeko ze-acidic;I-Salvianolic acid A kunye ne-C ingaba yi-tautomeric kwisisombululo.
Iinkcukacha
＞5%,＞10%,＞50%,＞70%,＞90%,98%
Inkqubo yokutsalwa
I-Radix Salviae Miltiorrhizae yatyunyuzwa, yafakwa kwitanki yokutsalwa, ifakwe ngokuphindwe ka-8 ubuninzi be-0.01mol / l hydrochloric acid ngobusuku, kwaye emva koko yaxutywa ngokuphindwe ka-14 umthamo wamanzi.Isisombululo esikhutshwe nge-percolated sihlanjululwa ngu-AB-8 ikholomu ye-resin macroporous.Okokuqala, elute nge 0.01mol/l hydrochloric acid ukususa ukungcola non adsorbed, kwaye ke elute nge 25% ethanol ukususa ukungcola kakhulu polar.Okokugqibela, gxininisa kwi-40% ye-ethanol eluent phantsi koxinzelelo oluncitshisiweyo lokubuyisela i-ethanol kunye ne-freeze-dry ukufumana iyonke iSalvia miltiorrhiza phenolic acid enobunyulu obungaphezulu kwe-80%.
Chonga
Thatha i-1g yemveliso, uyigaye, yongeza i-5ml ye-ethanol, ugxobhoze ngokupheleleyo, hluza, uthathe amathontsi ambalwa e-filtrate, uyifake kumgca wephepha lokucoca, uyomise, uyigcine phantsi kwesibane se-ultraviolet (365nm), bonisa i-blue- I-fluorescence engwevu, xhoma iphepha lokucoca kwibhotile yesisombululo se-ammonia (ungaqhagamshelani nomphezulu wolwelo), yikhuphe emva kwemizuzu engama-20, yijonge phantsi kwesibane se-ultraviolet (365nm), bonisa i-fluorescence eluhlaza okwesibhakabhaka.
Ubumuncu:thatha isisombululo samanzi phantsi kwento yokucaca, kwaye ixabiso le-pH liya kuba yi-2.0 ~ 4.0 (isihlomelo se-Chinese Pharmacopoeia 1977 Edition).
Ukumiselwa komxholo
Kwamiselwa yichromatography yolwelo oluphezulu olusebenzayo (Isihlomelo VI D, uMqulu I, i-Chinese Pharmacopoeia, 2000 EDITION).
I-Octadecyl silane edityanisiweyo ye-silica gel yasetyenziswa njenge-filler kwiimeko zechromatographic kunye novavanyo lwenkqubo yokusebenza;I-Methanol acetonitrile formic acid yamanzi (30:10:1:59) yayiyisigaba esihambayo;Ubude bokubona ubude be-286 nm.Inani leepleyiti zethiyori ezibalwe ngokwencopho ye-salvianolic acid B ayisayi kuba ngaphantsi kwe-2000.
Ukulungiswa kwesisombululo sereferensi ngokuchanekileyo umlinganiselo ofanelekileyo wesisombululo sesalathiso se-salvianolic acid B kunye nokongeza amanzi ukwenza ukuba iqulethe i-10% nge-1ml nganye ye-G isisombululo.
Ukulungiswa kwesisombululo sovavanyo kuthatha malunga ne-0.2g yemveliso, ukulinganisa ngokuchanekileyo, uyibeke kwibhotile yokulinganisa i-50ml, yongeza inani elifanelekileyo le-methanol, i-sonicate imizuzu engama-20, uyipholise, yongeza amanzi kwisikali, yihluthe kakuhle, ihluzo. it, ukulinganisa ngokuchanekileyo i-1ml ye-filtrate eqhubekayo, yifake kwibhotile yokulinganisa i-25ml, yongeza amanzi kwisikali, yigubungele kakuhle.
Indlela yokuzimisela ithatha ngokuchanekileyo i-20% yesisombululo solawulo kunye ne-20% yesisombululo sovavanyo μ l.Yifake kulwelo lwechromatograph ukuze uzimisele.
Ukusebenza kwe-Pharmacological
I-Salvianolic acid B kukuxinaniswa kweemolekyuli ezintathu zeDanshensu kunye nemolekyuli enye ye-caffeic acid.Yenye yeeasidi ze-salvianolic ezifundwe ngakumbi.Ineziphumo ezibalulekileyo ze-pharmacological kwintliziyo, ingqondo, isibindi, izintso kunye namanye amalungu.
I-Antioxidant
I-Salvianolic acid B inempembelelo enamandla ye-antioxidant.Iimvavanyo ze-vivo kunye ne-in vitro zibonisa ukuba i-salvianolic acid B inokukhupha i-oxygen free radicals kwaye inqanda i-lipid peroxidation.Ukuqina kwesenzo sayo kuphezulu kune-vitamin C, i-vitamin E kunye ne-mannitol.Yenye yeemveliso zendalo ezaziwayo ezinempembelelo enamandla ye-antioxidant Izifundo ze-Pharmacological zibonisa ukuba i-salvianolic acid yenaliti inesiphumo esicacileyo se-antioxidant, inqanda ukuhlanganiswa kweplatelet kunye ne-thrombosis, kwaye inokwandisa ixesha lokusinda kwezilwanyana phantsi kwe-hypoxia.Iziphumo zibonise ukuba i-salvianolic acid yenaliti (i-60 ~ 15mg / kg) inokuphucula kakhulu ukusilela kwe-neurological kwiigundane ezinokulimala kwe-cerebral ischemia-reperfusion, ukuphucula ukuphazamiseka kokuziphatha kunye nokunciphisa kakhulu indawo ye-cerebral infarction.Kwakukho umahluko omkhulu phakathi kwamanani aphezulu kunye aphakathi (60 kunye ne-30mg / kg);I-Salvianolic acid ye-injection inokuphucula kakhulu umonakalo we-neurological obangelwa yi-FeCl3 eyenza i-cerebral ischemia kwiigundane kwi-1, i-2 kunye ne-24 iiyure emva kokulawulwa, okubonakaliswa ekuphuculeni ukuphazamiseka kokuziphatha kunye nokunciphisa indawo ye-cerebral infarction;I-Salvianolic acid i-40 mg / kg ye-injection inqande kakhulu ukuhlanganiswa kweeplatelet zomvundla ezibangelwa yi-ADP, i-arachidonic acid kunye ne-collagen, kwaye amazinga okuthintela ayengama-81.5%, 76.7% kunye ne-68.9% ngokulandelanayo.I-Salvianolic acid 60 kunye ne-30mg / kg yenaliti inqande kakhulu i-thrombosis kwiimpuku;I-Salvianolic acid 60 kunye ne-30mg / kg yenaliti yandisa kakhulu ixesha lokusinda kweempuku phantsi kwe-hypoxia.
Isicelo seKlinikhi
Le mveliso ineempembelelo zokukhuthaza ukujikeleza kwegazi kunye nokususa i-stasis yegazi, i-dredging meridians kunye ne-activation collaterals.Isetyenziselwa ikakhulu ukunyanga i-ischemic stroke ebangelwa yi-blood stasis evalela i-meridians, efana nokuba ndindisholo kwesiqingatha somzimba kunye nemilenze, ubuthathaka, iintlungu zekhontrakthi, ukusilela kwemoto, ukuphambuka komlomo kunye namehlo, njl.
Gcina
Kwindawo epholileyo neyomileyo.
Ixesha lokuQinisekisa
Iminyaka emibini.
Indlela yokugcina
2-8 ° C, igcinwe kwindawo epholileyo neyomileyo kwaye kude nokukhanya.
Imibandela Efuna Ingqalelo
Imveliso kufuneka igcinwe kwiqondo lokushisa eliphantsi.Ukuba ibonakaliswe emoyeni ixesha elide, umxholo uya kuncitshiswa.
