Iimveliso

Typhonine glycoside

I-Puhuang yimpova eyomileyo yeTypha angustifolia L., iTypha Orientalis Presl okanye izityalo ezifanayo kwiTyphaceae.Umxholo we-isorhamnetin-3-o-neohesperidin kwi-Chinese Pharmacopoeia (uHlelo lwe-2005) lunqunywe yi-HPLC.Iindlela eziphambili zokumisela i-isorhamnetin-3-o-neohesperidin kwiTyphae kunye namalungiselelo ayo

HPLC.Phantsi kokumiselwa komxholo weTypha in Chinese Pharmacopoeia (uHlelo luka-2005):

Iimeko zeChromatographic kunye novavanyo lokusebenziseka kwenkqubo: i-octadecyl silane edibeneyo ye-silica gel njenge-filler;Amanzi e-acetonitrile (15:85) asetyenziswe njengesigaba esihambayo;Ubude bokubona ubude be-254 nm.Ubushushu bekholamu yi-40 ℃.Inani leeplati zethiyori aziyi kuba ngaphantsi kwe-1000 ngokwencopho ye-isorhamnetin-3-o-neohesperidin.

Ukulungiswa kwesisombululo sovavanyo: thatha malunga ne-0.5g yale mveliso, uyilinganise ngokuchanekileyo, uyibeke kwibhotile yokulinganisa i-50ml, yongeza i-45ml ye-methanol, unyango lwe-ultrasonic (amandla 250W, i-frequency 20KHz) imizuzu engama-30, yipholise, yongeza i-methanol isikali, yishukumise kakuhle, yihluze, kwaye uthathe isihluzo esiqhubekayo

Ukuzimisela kwe-isorhamnetin-3-o-neohesperidin ePuhuang kunye namalungiselelo ayo yi-HPLC.Inkqubo yesigaba esiphathwayo jikelele ngamanzi e-acetonitrile:

① Hp1050 ukusebenza okuphezulu kwechromatograph engamanzi;Ikholamu yeChromatographic C18 (5) μ m,4.6mm × 250mm) Dalian Institute of Chemical Physics, Chinese Academy of Sciences);Isigaba seselula: amanzi e-acetonitrile (15:85);Ukubona ubude bamaza: 254nm;Ubushushu bekholamu yegreenhouse.

② Ukusebenza okuphezulu kwechromatograph yolwelo yi-hp1050 inkqubo yeNkampani yeHewlett Packard eUnited States;Ikholamu yeChromatographic bondclone 10 C18 (3.9mm) × 300mm）; Isigaba seselula: i-acetonitrile yamanzi (80:20);ukufumanisa ubude be-254 nm, i-bandwidth 4 nm;ireferensi yobude be-550 nm, i-bandwidth 100 nm;ukuthoba 8.

UFu Daxu et al.Ukugqiba iziqulatho ze-typhoniflorin kunye ne-isorhamnetin-3-o-neohesperidin kwi-extract epheleleyo ye-flavone yeTypha nge-HPLC kunye ne-1100 chromatograph.Ikholamu yechromatographic yi-eclipse XdB C18 (250mm × 4.6mm, 5 μ m); Isigaba seselula: i-acetonitrile-0.5% i-acetic acid methanol gradient elution (0min: 15:78:7; 15min: 23:70:7);Ubude bokubona ubude yi-248 nm.Ukulungiswa kwesicatshulwa se-flavonoids esipheleleyo seTypha: i-50g yeTypha ikhutshwe nge-60% ye-ethanol (500ml) × 2), i-1H ​​ngexesha ngalinye, isicatshulwa sigxininiswe kwi-40 ℃, isicatshulwa sachithwa kunye ne-600ml yamanzi kunye ne-centrifuged.Intsalela yachithwa nge-300ml yamanzi kunye ne-centrifuged.Dibanisa isisombululo centrifugal, lungisa umthamo ukuya 1000ml ngamanzi, adsorb kuyo kwikholamu macroporous resin (150ml macroporous resin dry ubunzima 75g) kunye amaxesha 1.5 isixa ichiza ekrwada, elute nge 600ml amanzi kunye 450ml of 20% ukususa ethanol ukungcola, emva koko elute nge 600ml ye 50% ethanol, kwaye eluent ifunyanwa nge vacuum drying kwi 40 ℃.

Yang Yonghua et al.Ukusetyenziswa okuphezulu kwe-capillary electrophoresis kunye nokusebenza okuphezulu kolwelo lwechromatography ukumisela imixholo ye-isorhamnetin 232o2, neohesperidin kunye netyphoside ePuhuang.Isixhobo se-Hpce-3d esiphezulu se-capillary electrophoresis (i-Hewlett Packard, e-USA), ikholamu ye-quartz capillary 50 μ m × 56cm (HP), i-lc-6a i-chromatograph yolwelo oluphezulu (inkampani yaseShimadzu yaseJapan), i-C18 ikholamu 5mm × 200mm, ubukhulu be-particle 5 μ M (inkampani yaseDalian Yite).Iimeko ze-HPCE: i-buffer 0.02mol / l borax-0.05mol / l 10% i-acetonitrile ye-sodium dodecyl sulfate (SDS), i-voltage 20kV, ubushushu bekholamu 30 ℃, ubude bokubona i-270nm, umthamo we-injection 30KPa · s.Iimeko ze-HPLC: isigaba esihambayo sasiyi-acetonitrile yamanzi (15:85), ukushisa kwekholomu kwakuyi-25 ℃, kunye ne-wavelength yokufumanisa yayiyi-254 nm.

Typhaneoside Reference into

[igama]ityphonioside [1]

[Inombolo yeCAS]104472-68-6

[indlela yokubona]HPLC ≥ 98%

[Inkcazo]20mg 50mg 100mg 500mg 1g (inokupakishwa ngokweemfuno zomthengi)

[iipropati]umgubo we-amorphous