Verbascoside CAS No. 61276-17-3
Ulwazi oluBalulekileyo
[igama]Mullein glycoside
[isikweyeli]ergosterol, Mullein
[udidi]phenylpropanoid glycosides
[igama lesiNgesi]acteoside;I-Verbascoside;Kusaginin
[ifomula yemolekyuli]C29H36O15
[ubunzima bemolekyuli]624.59
[Inombolo yeCAS]61276-17-3
IiPropati zePhysicochemical
[iipropati]le mveliso iyinaliti emhlophe yomgubo wekristale
[ubuninzi obuzalanayo]1.6g/cm3
[ukunyibilika]Inyibilika ngokulula kwi-ethanol, i-methanol kunye ne-ethyl acetate.
Umthombo wokutsalwa
Le mveliso sisikhondo esomileyo esinamagqabi anamaxolo eCistanche deserticola, isityalo sosapho lwe liedang.
Indlela yoVavanyo
HPLC ≥ 98%
Iimeko zeChromatographic: i-mobile phase methanol acetonitrile 1% i-acetic acid (15:10:75), izinga lokuhamba kwe-0.6 ml · min-1, ubushushu bekholamu 30 ℃, ubude be-334 nm (ukukhangela kuphela)
Umsebenzi kunye nokusetyenziswa
Le mveliso isetyenziselwa ukumisela umxholo
Indlela yokugcina
2-8 ° C, igcinwe kude nokukhanya.
I-Bioactivity ye-Verbascoside
Isifundo se-In Vitro:
Njengokhuphiswano lwe-PKC Inhibitor ye-ATP, i-Verbascoside ine-IC50 ye-25 μ M. I-Verbascoside ibonise i-kis ye-22 kunye ne-28 malunga ne-ATP kunye ne-histone, ngokulandelanayo μ M [1]。 I-Verbascoside (5,10) μ M) Uthintelo lwe-2,4-dinitrochlorobenzene (DNCB) - i-T cell costimulatory factor i-CD86 kunye ne-CD54, i-proinflammatory cytokines kunye ne-NF kwiiseli ze-thk-1 κ B indlela yokusebenza [2].
Kwizifundo zeVivo:
I-Verbascoside (1%) yanciphisa iziganeko zokuziphatha okukhatywayo ngokubanzi kunye nobukhulu bezilonda zesikhumba kwimodeli ye-mouse ye-2,4-dinitrochlorobenzene (DNCB) - i-atopic dermatitis (AD).I-Verbascoside inokuthintela i-cytokine ye-pro-inflammatory TNF kwi-DNCB eyenza izilonda zesikhumba- α, Ukubonakaliswa kwe-IL-6 kunye ne-IL-4 mRNA [2].I-Verbascoside (50100 mg / kg, IP) ayizange itshintshe intlungu ebandayo engaqhelekanga ebangelwa ukulimala okungapheliyo (CCI).I-Verbascoside (i-200 mg / kg, i-IP) yanciphisa ukungezwani komzimba kwi-acetone evuselelayo ebandayo ngosuku lwe-3. I-Verbascoside nayo yanciphisa kakhulu utshintsho lokuziphatha oluhambelana ne-neuropathy.Ukongezelela, i-Verbascoside yanciphisa i-Bax kwaye yanda i-Bcl-2 ngosuku lwe-3 [3].
Uvavanyo lweSeli:
i-lymphocytic mouse leukemia iiseli ze-L1210 (ATCC, CCL 219) zine-10% ye-fetus bovine serum, 4 mM glutamine, 100 U / ml penicillin, 100 μ Kuluhlu lwamaqula angama-24 epleyiti elungisiweyo yoKhozi, iiseli ezili-104 kwiqula ngalinye zazimbalwa. I-Ml streptomycin sulfate kunye ne-Verbascoside (echithwe kwi-DMSO).Ukukhula kwajongwa ngokubala inani leeseli kwikhawuntara ye-Coulter emva kweentsuku ezi-2 zokufukamela kwindawo efumileyo (5% CO2 emoyeni) kuma-37 ℃.Ixabiso le-IC50 libalwe ngokusekelwe kumgca wokubuyisela umgca osekwe kwikhompawundi nganye yovavanyo [1].
Uvavanyo lwezilwanyana:
Ukuze kubangele i-atopic dermatitis (AD) - njengeempawu, iigundane [2] zisetyenziselwa i-2,4-dinitrochlorobenzene (DNCB).Ngamafutshane, inwele ye-dorsal yeegundane yasuswa kunye ne-scissors ye-elektroniki kwiintsuku ze-2 ngaphambi kokuba unyango lwe-DNCB.Ngaba i-200 μ L ye-1% ye-DNCB (kwi-acetone: ioli yeoli = 4: 1) isetyenziswe kwisikhumba esichetyiweyo sokuvuselela.Ukuhlaselwa okuphindaphindiweyo kwenziwa kwindawo efanayo, i-0.2% ye-DNCB yonke imihla ye-3 malunga neeveki ze-2.Iigundane zahlulwa zibe ngamaqela e-4 (n = 6 kwiqela ngalinye): (1) ulawulo oluphathwayo lwesithuthi, (2) i-DNCB iphathwa kuphela, (3) 1% Verbascoside (i-acetone: ioli yeoli 4: 1) - iphathwa kuphela, kwaye ( 4) DNCB + 1% Verbascoside iqela eliphathwayo[2].
Isalathiso:
[1].UHerbert JM, et al.I-Verbascoside eyedwa kwiLantana camara, inhibitor yeprotein kinase C. J Nat Prod.1991 Nov-Dec;54(6):1595-600.
[2].ULi Y, kunye nabanye.I-Verbascoside Yehlisa i-Atopic Dermatitis-Njengeempawu kwiiMice ngokusebenzisa i-Anti-Inflammatory Effect yayo enamandla.I-Int Allergies Immunol.2018;175(4):220-230.
[3].U-Amin B, kunye no-al.Umphumo we-Verbascoside kwi-Pain ye-Neuropathic ebangelwa yi-Chronic Constriction Injury kwiiRats.Phytother Res.2016 Jan; 30 (1): 128-35.
