Jujuboside A CAS No.. 55466-04-1
Alaye pataki
Inagijẹ Kannada:jujube ekuro saponin A, jujube ekuro saponin A;Jujube ekuro saponin A (boṣewa)
Orukọ Gẹẹsi:Jujuboside A
Orisun ọgbin:o wa lati inu awọn irugbin jujube igbẹ, ti a tun mọ ni jujube ekuro ati mojuto jujube egan
Ilana molikula:C58H94O26
Ìwúwo molikula:1207.35
CAS No.55466-04-1
Bioactivity ti Jujuboside A
Àfojúsùn:GABA olugba, mTOR, PI3K, Akt [1] [2]
Iwadi inu vitro:iwọn kekere 41 μ Jujuboside A ti M (nipa 0.05 g / L) ti o fa GABA (a) awọn olugba lakoko itọju 24 ati 72 h α 1, α 5, β 2 subunit mRNA pọ si ni pataki.Iwọn giga 82 μM (nipa 0.1 g / L) Jujuboside A pọ si GABA (a) awọn olugba ni awọn ipele 24 h α 1, α 5 subunit mRNA ipele ati dinku β 2 subunit mRNA ipele ati dinku GABA (a) subunit α 1 ， A ṣe itọju ikosile β2 mRNA ni 72 h [1].Jujuboside Itọju iṣaaju le yi idinku idinku ti ṣiṣeeṣe sẹẹli pada ki o si fa ipalara ISO ti o dara julọ fa ipalara sẹẹli H9c2.Jujuboside A le mu yara phosphorylation ti PI3K, Akt ati mTOR.Jujuboside A le dinku ni pataki ipin ti amuaradagba ti o somọ microtubule LC3-II / I ninu awọn sẹẹli H9c2 [2]
Iwadi ninu vivo:lakoko ọjọ (9: 00-15: 00), awọn jujubosides pọ si pọsi lapapọ oorun ati gbigbe oju iyara (REM) oorun, ṣugbọn ko ni ipa pataki lori oorun ti kii REM (NREM).Ni alẹ (21: 00-3: 00), awọn jujubosides pọ si pupọ oorun lapapọ ati oorun NREM, paapaa oorun kekere, ṣugbọn ko ni ipa pataki lori oorun REM ati oorun igbi ti o lọra (SWS) [3].Itọju inu ventricular pẹlu jjuboside a ti dinku ni pataki nipasẹ a nipasẹ Y-maze, yago fun ṣiṣe ati awọn wiwọn iruniloju omi Morris β 1-42 ti o fa ẹkọ ati ailagbara iranti ninu awọn eku.Abẹrẹ intracerebroventricular ti Jujuboside A dinku hippocampal a β 1-42 awọn ipele pataki ṣe idiwọ awọn iṣẹ ti acetylcholinesterase (AChE) ati rara, ati dinku akoonu ti o pọ si ti malondialdehyde (MDA) ninu hippocampus ati kotesi cerebral ti eku ti a fi sinu ventricle 1-42 beta ti ita. [4].
Idanwo sẹẹli:lẹhin awọn ọjọ 7 ti aṣa ni fitiro, awọn sẹẹli naa ti farahan nigbagbogbo fun wakati 24 tabi awọn wakati 72, ati pe wọn ko farahan si alabọde ti o ni Jujuboside A tabi diazepam tabi mejeeji.A ṣe afikun Vector si ẹgbẹ iṣakoso;Ẹgbẹ Diazepam fi kun 10 μ M diazepam;Jujuboside A82 μM (nipa 0.1g / L) ati 41 μM (nipa 0.05 g / L) ni a fi kun si jua-h (iwọn lilo giga Jujuboside A) ati jua-l (iwọn kekere Jujuboside A) awọn ẹgbẹ, lẹsẹsẹ.Lapapọ RNA ti ya sọtọ si awọn sẹẹli fun itupalẹ siwaju [1].
Idanwo ẹranko:eku: a jẹ itasi nipasẹ ICV β 1-42 ti o fa ailagbara oye ninu awọn eku.Lẹhinna, awọn eku ni abẹrẹ pẹlu Jujuboside A (0.02 ati 0.2 mg / kg) intraventricular (ICV) fun awọn ọjọ 5.Iruniloju Y, yago fun ṣiṣe ati awọn idanwo iruniloju omi Morris ni a ṣe lori awọn eku [1].
Inagijẹ Gẹẹsi ti Jujuboside A
α-L-arabinopyranoside, (3β,16β,23R) -16,23:16,30-diepoxy-20-hydroxydammar-24-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1- > 2)-O-[O-β-D-glucopyranosyl- (1-> 6)-O-[β-D-xylopyranosyl-(1-> 2)] -β-D-glucopyranosyl- (1->3) )]-
(3β,16β,23R) -20-Hydroxy-16,23:16,30-diepoxydammar-24-en-3-yl 6-deoxy-α-L-mannopyranosyl-(1->2)-[β-D -glucopyranosyl- (1-> 6)-[β-D-xylopyranosyl- (1-> 2)] -β-D-glucopyranosyl- (1-> 3)] -α -L-arabinopyranoside.
jujuboside C
α-L-Arabinopyranoside, (3β,16β,23R) -16,23:16,30-diepoxy-20-hydroxydammar-24-en-3-yl O-6-deoxy-α-L-mannopyranosyl-(1- > 2)-O-[O-β-D-glucopyranosyl- (1-> 6)-O-[β-D-xylopyranosyl- (1-> 2)] - β-D-glucopyranosyl- (1-> 3) )]-
JujubosideA
(3β,16β,23R) -20-Hydroxy-16,23:16,30-diepoxydammar-24-en-3-yl 6-deoxy-α-L-mannopyranosyl-(1->2)-[β-D -glucopyranosyl- (1-> 6)-[β-D-xylopyranosyl- (1-> 2)] -β-D-glucopyranosyl- (1-> 3)] -α-L-arabinopyranoside.
