Paeoniflorin CAS No.. 23180-57-6
Alaye pataki
Bakannaa mọ bi paeoniflorin, o jẹ glycoside kikoro pinane monoterpene ti o ya sọtọ lati peony pupa ati peony funfun.O jẹ lulú amorphous hygroscopic.O wa ninu awọn gbongbo ti Paeonia, peony, peony eleyi ti ati awọn irugbin miiran ti Ranunculaceae.Majele ti okuta kirisita yii kere pupọ.
[orukọ kemikali]5beta-[(Benzoyloxy) methyl] tetrahydro-5-hydroxy-2-methyl-2,5-methano-1H-3,4-dioxacyclobuta[cd] pentalen-1alpha (2H) -yl-beta-D-glucopyranoside
[ agbekalẹ molikula]C23H28O11
【CASRara】23180-57-6
Mimọ: loke 98%, ọna wiwa: HPLC.
[orisun]awọn gbongbo ti Paeonia albiflora pall, P. suffrsticosa Andr, P. delarayi Franch, ohun ọgbin ti Ranunculaceae, akoonu ti radix paeoniae Rubr jẹ ti o ga julọ.
[Pato]10%, 20%, 30%, 50%, 90%, 98%
[Anṣiṣẹ lọwọIeroja ] Lapapọ awọn glucosides ti Paeonia (TGP) jẹ orukọ gbogbogbo ti paeoniflorin, hydroxy paeoniflorin, paeoniflorin, albiflorin ati benzoyl paeoniflorin, eyiti a pe ni TGP fun kukuru.
Ti ara ati kemikali-ini
O jẹ hygroscopic amorphous Tan lulú (90% ti wa ni pipa funfun lulú) [α] 16D-12.8. (C = 4.6, methanol), tetraacetate jẹ kirisita acicular ti ko ni awọ, aaye yo: 196 ℃.Paeoniflorin jẹ iduroṣinṣin ni agbegbe ekikan (pH 2 ~ 6) ati riru ni agbegbe ipilẹ.
Ipinnu akoonu
Ni gbogbogbo, ọna 1 ati ọna 2 tun le ṣee lo fun wiwa.Ọna 1 jẹ lilo ti o dara julọ fun iṣelọpọ akoonu giga, eyiti o le ṣe iranlọwọ fun oṣiṣẹ ilana ti o dara lati ṣe idajọ mimọ ti awọn ọja.Nkan itọkasi jẹ rọrun lati decompose lẹhin itusilẹ.
1.It ti pinnu nipasẹ iṣẹ ṣiṣe giga chromatography omi (Afikun VI d).Awọn ipo chromatographic ati ibamu eto ni idanwo pẹlu Octadecyl silane bonded silica gel bi kikun;Acetonitrile-0.1% ojutu phosphoric acid (14: 86) ni a lo bi alakoso alagbeka;Gigun oju wiwa jẹ 230nm.Nọmba awọn awo-ara ti a ṣe iṣiro ni ibamu si paeoniflorin tente oke ko yẹ ki o kere ju 2000. Igbaradi ti ojutu itọkasi: ni deede ṣe iwọn iye ti o yẹ ti ojutu itọkasi paeoniflorin ati ṣafikun methanol lati mura 60% paeoniflorin fun ojutu 1ml μG.
2.Lati ṣe atunṣe ọna ipinnu ti paeoniflorin ni Radix Paeoniae Alba.Awọn ọna: awọn ọna ti o wa ni Kannada Pharmacopoeia ati awọn ọna ilọsiwaju ti a ṣe afiwe.Ipele alagbeka jẹ omi kẹmika kẹmika (30:70) ati iwọn gigun wiwa jẹ 230nm.esi;Ibasepo laini ti ọna yii dara (r = 0.9995).Imularada apapọ jẹ 101.518% ati RSD jẹ 1.682%.Ipari: ọna ti o ni ilọsiwaju jẹ rọrun ati deede, eyi ti o le dinku majele ti awọn nkan ti o wa ni erupẹ si awọn eniyan ati idoti ayika, ati pese ipilẹ itọkasi fun ipinnu paeoniflorin ni iṣe.
Ọna Ipinnu
Ipinnu paeoniflorin nipasẹ HPLC
Ààlà ohun elo:ọna yii nlo HPLC lati pinnu akoonu ti paeoniflorin ninu awọn oogun Guizhi Fuling.
Ọna naa dara fun oogun Guizhi Fuling.
Ilana Ilana:fi ayẹwo idanwo sinu ọpọn conical, ṣafikun iye ti o yẹ ti ethanol dilute fun isediwon ultrasonic, tutu, gbọn daradara, ṣe àlẹmọ, filtrate wọ inu chromatograph omi iṣẹ giga fun ipinya chromatographic, lo aṣawari gbigba ultraviolet lati rii Iye gbigba ti paeoniflorin ni gigun ti 230nm, ati ṣe iṣiro akoonu rẹ.
