Awọn ọja

Typhonine glycoside

Puhuang jẹ eruku adodo ti o gbẹ ti Typha angustifolia L., Typha Orientalis Presl tabi awọn ohun ọgbin ti o jọra ni Typhaceae.Awọn akoonu ti isorhamnetin-3-o-neohesperidin ni Chinese Pharmacopoeia (2005 Edition) jẹ ipinnu nipasẹ HPLC.Awọn ọna akọkọ fun ipinnu isorhamnetin-3-o-neohesperidin ni Typhae ati awọn igbaradi rẹ jẹ

HPLC.Labẹ ipinnu akoonu ti Typha ni Pharmacopoeia Kannada (Ẹya 2005):

Awọn ipo Chromatographic ati idanwo ohun elo eto: octadecyl silane bonded silica gel as filler;Omi Acetonitrile (15:85) ni a lo gẹgẹbi apakan alagbeka;Gigun oju wiwa jẹ 254 nm.Iwọn otutu 40 ℃.Nọmba awọn awo-ara imọ-jinlẹ ko ni kere ju 1000 ni ibamu si tente oke ti isorhamnetin-3-o-neohesperidin.

Igbaradi ojutu idanwo: mu nipa 0.5g ti ọja yii, ṣe iwọn deede, fi sinu igo wiwọn 50ml, fi 45ml ti methanol, itọju ultrasonic (agbara 250W, igbohunsafẹfẹ 20KHz) fun awọn iṣẹju 30, tutu, ṣafikun methanol si asekale, mì o daradara, àlẹmọ o, ati ki o ya awọn lemọlemọfún ase

Ipinnu ti isorhamnetin-3-o-neohesperidin ni Puhuang ati awọn igbaradi rẹ nipasẹ HPLC.Eto alakoso alagbeka gbogbogbo jẹ omi acetonitrile:

① Hp1050 iṣẹ ṣiṣe giga chromatograph;Chromatographic iwe C18 (5) μ m,4.6mm × 250mm) Dalian Institute of Chemical Physics, Chinese Academy of Sciences);Abala alagbeka: omi acetonitrile (15:85);Gigun wiwa: 254nm;Oju ewe eefin otutu.

② Awọn chromatograph omi ti o ga julọ jẹ eto hp1050 ti Ile-iṣẹ Hewlett Packard ni Amẹrika;Chromatographic iwe bondclone 10 C18 (3.9mm) × 300mm);wiwa wefulenti 254 nm, bandiwidi 4 nm;itọkasi wefulenti 550 nm, bandiwidi 100 nm;attenuation 8.

Fu Daxu et al.Ti pinnu awọn akoonu ti typhoniflorin ati isorhamnetin-3-o-neohesperidin ni apapọ flavone jade ti Typha nipasẹ HPLC ati 1100 chromatograph.Oju-iwe chromatographic jẹ oṣupa XdB C18 (250mm × 4.6mm, 5 μ m) ； Ilana alagbeka: acetonitrile-0.5% acetic acid methanol gradient elution (0min: 15: 78: 7; 15min: 23: 70: 7);Gigun wiwa wiwa jẹ 248 nm.Igbaradi ti lapapọ flavonoids jade ti Typha: 50g ti Typha ti a fa jade pẹlu 60% ethanol (500ml) × 2), 1H kọọkan akoko, awọn jade ti a ogidi ni 40 ℃, awọn jade ti a ni tituka pẹlu 600ml omi ati centrifuged.Iyoku ti tu pẹlu 300ml ti omi ati centrifuged.Darapọ ojutu centrifugal, ṣatunṣe iwọn didun si 1000ml pẹlu omi, adsorb lori iwe ti resini macroporous (150ml macroporous resin gbẹ iwuwo 75g) pẹlu awọn akoko 1.5 iye oogun robi, elute pẹlu 600ml ti omi ati 450ml ti 20% ethanol lati yọkuro awọn aimọ, lẹhinna elute pẹlu 600ml ti 50% ethanol, ati pe eluent ni a gba nipasẹ gbigbẹ igbale ni 40 ℃.

Yang Yonghua et al.Ti a lo electrophoresis capillary iṣẹ giga ati kiromatogirafi olomi iṣẹ giga lati pinnu awọn akoonu ti isorhamnetin 232o2, neohesperidin ati typhoside ni Puhuang.Hpce-3d iṣẹ giga capillary electrophoresis irinse (Hewlett Packard, USA), quartz capillary column 50 μ m × 56cm (HP), lc-6a iṣẹ giga ti omi chromatograph (Shimadzu ile ti Japan), C18 iwe 5mm × 200mm, patiku iwọn 5 μ M (ile-iṣẹ Dalian Yite).Awọn ipo HPCE: saarin 0.02mol/l borax-0.05mol/l 10% acetonitrile of sodium dodecyl sulfate (SDS), foliteji 20kV, iwe otutu 30 ℃, erin wefulenti 270nm, abẹrẹ iwọn didun 30KPa · s.Awọn ipo HPLC: apakan alagbeka jẹ omi acetonitrile (15:85), iwọn otutu ọwọn jẹ 25 ℃, ati igbi wiwa jẹ 254 nm.

Ohun elo Itọkasi Typhaneoside

[orukọ]typhonioside [1]

[CAS No.]104472-68-6

[ọna idanimọ]HPLC ≥ 98%

[Pato]20mg 50mg 100mg 500mg 1g (le ṣe akopọ gẹgẹbi awọn ibeere alabara)

[ohun-ini]amorphous lulú