I-Astragaloside IV CAS No. 84687-43-4
Isingeniso esifushane
Isibizo sesiNgisi:I-Astragaloside IV;i-beta-D-Glucopyranoside, (3beta,6alpha,16beta,24R)-20,24-epoxy-16,25-dihydroxy-3-(beta-D-xylopyranosyloxy) -9,19-cyclolanostan-6-yl;(3beta,6alpha,9beta,16beta,20R,24S)-16,25-dihydroxy-3-(beta-D-xylopyranosyloxy)-20,24-epoxy-9,19-cyclolanostan-6-yl beta-D-threo - i-hexopyranoside
I-Molecular Formula:I-C41H68O14
Igama Lekhemikhali:I-17-[5-(1-Hydroxyl-1-methyl-ethyl)- 2methyl-tetrahydro- furan-2-yl]-4,4,13,14-tetramethyl-tetradecahydro-cyclopropa[9,10]cyclopenta[a] phenanthren-16-ol-3-β-D-aracopyranosyl-6-β-D-glucoside
Mp:200 ~ 204 ℃
[α]D:-56.6(c,0.13 ku-DMF)
UV:λmax203 nm
ubumsulwa:98%
Umthombo:i-legume Astragalus membranaceus, i-Astragalus pubescens.
Ifomula yesakhiwo sekhemikhali ye-astragaloside IV
Izakhiwo ze-Physicochemical
[ukubukeka]:i-crystalline powder emhlophe
[ukuhlanzeka]:ngaphezu kwama-98%, indlela yokuthola: HPLC
[umthombo wesitshalo]:izimpande zika-Astragalus Alexandrinus Boiss, Astragalus dissectus, Astragalus membranaceus (Fisch.) I-Bungede impande, i-Astragalus sieversianus Pall Root ye-Astragalus spinosus Vahl, ingxenye yasemoyeni ye-Astragalus spinosus Vahl.
[izakhiwo zomkhiqizo]:I-Astragalus membranaceus ekhishwe yimpushana ephuzi ensundu.
[ukunqunywa kokuqukethwe]:kunqunywa yi-HPLC (Isithasiselo VI D, uMqulu I, i-Chinese Pharmacopoeia, Uhlelo luka-2010).
Izimo ze-Chromatographic nokuhlolwa kokusebenza kwesistimu} ijeli ye-silica eboshiwe ye-octadecyl silane isetshenziswa njengesigcwalisi, amanzi e-acetonitrile (32:68) asetshenziswa njengesigaba esihambayo, futhi umtshina ohlakazekayo wokukhanya usetshenziselwa ukutholwa.Inani lamapuleti etiyori akufanele libe ngaphansi kuka-4000 ngokuya ngenani eliphakeme le-astragaloside IV.
Ukulungiswa kwesixazululo sereferensi, thatha inani elifanele lereferensi ye-astragaloside IV, uyikale ngokunembile, bese wengeza i-methanol ukuze ulungise isisombululo esiqukethe i-0.5mg nge-1ml ngayinye.
Ukulungiswa kwesixazululo sokuhlola:thatha cishe i-4G yempushana kulo mkhiqizo, uyikale kahle, uyifake ku-Soxhlet extractor, engeza u-40ml we-methanol, uyicwilise ubusuku bonke, engeza inani elifanele le-methanol, ukushisa kanye ne-reflux amahora angu-4, buyisela isincibiliki esiphuma esikhiqizweni bese ugxilisa ingqondo. ukuze yome, engeza u-10ml wamanzi ukuze uncibilikise insalela, unyakazise futhi uyikhiphe nge-n-butanol egcwele izikhathi ezingu-4, 40ml isikhathi ngasinye, hlanganisa isixazululo se-n-butanol, futhi uyigeze ngokugcwele ngesisombululo sokuhlola i-ammonia izikhathi ezi-2, 40ml ngayinye. isikhathi, lahla isixazululo se-ammonia, uhwamulise isixazululo se-n-butanol, engeza amanzi angu-5ml ukuze uncibilikise insalela, futhi uyipholise, Ngekholomu ye-resin ye-adsorption engu-D101 (ububanzi bangaphakathi: 37.5px, ukuphakama kwekholomu: 300px), i-elite ngamanzi angu-50ml , lahla isixazululo samanzi, i-ethanol engu-30ml ka-40%, lahla i-ethanol, i-ethanol engu-80ml ka-70% ethanol, qoqa i-ethanol, uyihwamulise ukuze yome, ncibilikisa insalela nge-methanol, uyidlulisele ku-5ml volumetric flask, engeza. methanol esikalini, shake kahle, futhibese uyayithola.
Indlela yokunquma:amunca ngokunembile u-10% wesixazululo sereferensi ngokulandelana μ l, 20 μ l.Isixazululo sokuhlola 20 ngayinye μ l.Ijove ku-chromatograph ewuketshezi, inqume, bese uyibala ngesibalo se-logarithm yendlela yangaphandle yamaphoyinti amabili ajwayelekile.
Kubalwa njengomkhiqizo owomile, okuqukethwe kwe-astragaloside IV (c41h68o14) akufanele kube ngaphansi kuka-0.040%
Isenzo se-Pharmacological
Izingxenye ezisebenzayo ze-Astragalus yi-polysaccharides ne-astragaloside.I-Astragaloside ihlukaniswe yaba yi-astragaloside I, i-astragaloside II ne-astragaloside IV.Phakathi kwazo, i-astragaloside IV, i-astragaloside IV, inomsebenzi omuhle kakhulu webhayoloji.I-Astragaloside IV ayinawo umphumela we-Astragalus polysaccharides, kodwa futhi neminye imiphumela engenakuqhathaniswa ye-Astragalus polysaccharides.Ukuqina kwayo kokusebenza kungaphezu kokuphindwe kabili kokwejwayelekile kwe-astragalus polysaccharides, futhi umphumela wayo wokulwa namagciwane uphindwe izikhathi ezingama-30 kune-Astragalus polysaccharides.Ngenxa yokuqukethwe kwayo okuphansi nomphumela omuhle, yaziwa nangokuthi "i-super astragalus polysaccharide".
1.Thuthukisa ukuzivikela kanye nokungazweli kwezifo.
Ingakwazi ukufaka ngaphandle ngokuqondile nangokungaqondile imizimba yangaphandle ehlasela umzimba, ikhuthaze ukuzivikela okuqondile, ukuzivikela komzimba kanye nokungaqondile, futhi ithuthukise ukumelana nezifo komzimba.Ingakhuthaza ukukhiqizwa kwamasosha omzimba, futhi ithuthukise ngokuphawulekayo inani lamaseli akha amasosha omzimba kanye nenani lokuhlola i-hemolysis.I-Astragaloside IV ingathuthukisa kakhulu izinga lokuguqulwa kwe-lymphocyte kanye nezinga lokwakheka kwe-E-rosette yezinkukhu ezigonywe i-coccidia.Kuyi-activator ephumelelayo ye-monocyte macrophage system.I-Astragaloside IV ingabuye ithuthukise i-oxidation, imisebenzi ye-GSH-Px kanye ne-SOD ezithombeni zomzimba, futhi ithuthukise ukuzivikela komzimba kanye nemisebenzi yokuqapha amasosha omzimba.
2.Umphumela we-Antiviral.
Umgomo wayo wokulwa namagciwane: ukuvuselela ukusebenza kwama-macrophage nama-T cell, ukwandisa isibalo samaseli akha ama-E-ring, ukunxenxa ama-cytokines, ukukhuthaza ukungeniswa kwe-interleukin, nokwenza umzimba wesilwane ukhiqize i-interferon engapheli, ukuze ufeze inhloso ye-antiviral.Imiphumela yabonisa ukuthi inani eliphelele lokuvikela le-astragaloside IV ku-IBD lalingu-98.33%, elingavimbela ngempumelelo futhi liphathe i-IBD, futhi kwakungekho umehluko ophawulekayo uma kuqhathaniswa nesisombululo esiphezulu se-immune egg yolk.I-Astragaloside ingathuthukisa ukusebenza kwama-enzyme e-antioxidant emzimbeni, inciphise okuqukethwe kwe-LP0, inciphise ukulimala kwezinhlobo zelashwe we-oxygen esebenzayo, ngaleyo ndlela inciphise izinga lokufa nokufa kwe-MD.Ingathuthukisa ukuzivikela okuphansi okubangelwa isimila, ikhuthaze ukusebenza kwamangqamuzana omzimba omzimba, ikhulule izici ezingapheli, futhi ivimbele ukubulawa nokuvinjelwa kwamangqamuzana e-tumor okubangelwa i-peroxidation;I-Astragaloside a ingavimbela ukukhula kwegciwane lomkhuhlane kanye nomsebenzi we-sialidase.Kunomthelela omkhulu emsebenzini we-influenza virus cell membrane kanye nokukhangiswa kanye nokungena kwegciwane kumaseli azwelayo.Izinga lokufa kanye neqanda lokubeka amaqanda ezinkukhu lancipha kakhulu, futhi izinga lokubeka amaqanda kanye nokuvuselelwa kwekhwalithi yeqanda kwakungcono kakhulu kuneqembu le-amantadine kuphela lokulawula, futhi umphumela we-Astragalus polysaccharide wawungabonakali;I-Astragaloside IV inokubulala okuqinile kanye nemiphumela evimbelayo kugciwane.Isisekelo siwukuthi ukusetshenziswa kwe-astragaloside IV ngaphambi kokutholakala kokutheleleka ngegciwane le-Nd, ngakho-ke kungcono ukusebenzisa i-astragaloside IV isikhathi eside, i-Avian myeloblastic leukemia (AMB) yezinsuku ezingu-3 ubudala ye-AA broilers idliswe i-astragaloside IV nge-infection. yegciwane le-AMB, linganciphisa izinga lokushona kanye nokufa kwe-AMB, likhulise okuqukethwe kwe-LPO ezithweni zokuzivikela ezinjengobende kanye ne-thymus, lithuthukise kakhulu umphumela wokukhukhula we-spleen kanye ne-thymus nezinye izitho zokuzivikela ezifweni kumaseli e-myeloid asuselwa ku-tumor.Okwesibili, i-astragaloside IV inemiphumela esobala yokuvimbela kanye nokwelapha ezifweni zokuphefumula ezifana ne-laryngotracheitis ethathelwanayo.Sebenzisa.
3. Umphumela wokulwa nokucindezeleka.
I-Astragaloside IV ingavimbela i-adrenal hyperplasia kanye ne-thymus atrophy esikhathini esiyisixwayiso sokuphendula kokucindezeleka, futhi ivimbele izinguquko ezingavamile esikhathini sokumelana nenkathi yokwehluleka kokuphendula kwengcindezi, ukuze idlale indima yokulwa nokucindezeleka, ikakhulukazi inomthethonqubo obalulekile wezindlela ezimbili. umphumela kuma-enzyme enqubweni ye-metabolism yezakhi, futhi unciphisa futhi uqede umthelela wokucindezeleka kokushisa emsebenzini wokuphila komzimba ngezinga elithile.
4. Njengomgqugquzeli wokukhula.
Ingathuthukisa i-physiological metabolism yamangqamuzana, ithuthukise ukujikeleza kwegazi, ithuthukise umzimba wezilwane, futhi idlale indima yokondleka kanye nokunakekelwa kwezempilo.Ucwaningo lubonisa ukuthi ingakhuthaza ukukhula kwe-bifidobacteria kanye ne-lactic acid bacteria futhi inomphumela wama-probiotics.
5. I-Astragaloside IV ingathuthukisa umsebenzi we-cardiopulmonary.
Qinisa isivumelwano senhliziyo, vikela i-myocardium futhi umelane nokuhluleka kwenhliziyo.Ibuye ibe nemiphumela yokuvikela isibindi, i-anti-inflammatory kanye ne-analgesic.Ingasetshenziswa njenge-adjuvant therapy yezifo ezihlukahlukene ezibangelwa amagciwane namagciwane.
