I-Galangin CAS No. 548-83-4
Izakhiwo zomzimba namakhemikhali
Isibizo:I-Gaoliang Curcumin;3,5,7-trihydroxyflavone,
Igama lesiNgisi:galangin,
Isibizo sesiNgisi:3,5,7-trihydroxyflavone;3,5,7-trihydroxy-2-phenylchromen-4-one
Isakhiwo samangqamuzana
1. Inkomba ye-Molar refractive: 69.55
2. Ivolumu ye-Molar (m3 / mol): 171.1
3. Ivolumu eqondile ye-Isotonic (90.2k): 519.4
4. Ukushuba kobuso (dyne / cm): 84.9
5. I-polarizability (10-24cm3): 27.57
I-Computational Chemistry
1. Inani eliyisethenjwa lesibalo sepharamitha ye-hydrophobic (xlogp): Akukho
2. Inani labanikeli bebhondi ye-hydrogen: 3
3. Inombolo yama-hydrogen bond receptors: 5
4. Inombolo yamabhondi amakhemikhali ashintshayo: 1
5. Inani lama-tautomer: 24
6. I-Topological molecular polarity surface 87
7. Inani lama-athomu asindayo: 20
8. Ukushaja okungaphezulu: 0
9. Ukuba yinkimbinkimbi: 424
10. Inombolo yama-athomu e-isotopic: 0
11. Thola inani lama-atomic stereocenters: 0
12. Inombolo ye-atomic stereocenters engaqinisekile: 0
13. Nquma inani le-chemical bond stereocenters: 0
14. Inombolo ye-stereocenter yebhondi yamakhemikhali engapheli: 0
15. Inombolo yamayunithi ebhondi ehlangene: 1
Isenzo se-Pharmacological
I-Galangin ingaguqula i-Salmonella typhimurium TA98 ne-TA100 futhi inomphumela wokulwa namagciwane.
Isifundo se-In Vitro
I-Galangin ivimbe i-catabolism ye-DMBA ngendlela encike kumthamo.I-Galangin iphinde yavimbela ukwakheka kwe-DMBA-DNA adducts futhi yavimbela ukuvimbela ukukhula kwamaseli kwe-DMBA.Kumaseli angaguquki nama-microsomes ahlukanisiwe kumaseli alashwayo e-DMBA, i-galangin ikhiqize ukuvinjelwa okusebenzayo okuncike kudosi komsebenzi we-CYP1A1 olinganiswa umsebenzi we-ethoxypurine-o-deacetylase.Ukuhlaziywa kwe-inhibition kinetics nge-double reciprocal diagram ibonise ukuthi i-galangin ivimbele umsebenzi we-CYP1A1 ngendlela engaqhudelani.I-Galangin iholela ekwenyukeni kwezinga le-CYP1A1 mRNA, ebonisa ukuthi ingase ibe i-agonist ye-aromatic hydrocarbon receptor, kodwa ivimbela i-CYP1A1 mRNA (TCDD) eyenziwa yi-DMBA noma i-2,3,5,7-tetrachlorodibenzo-p-dioxin.I-Galangin iphinde ivimbele ukulotshwa okwenziwe i-DMBA noma i-TCDD kwamavektha wezintatheli aqukethe umgqugquzeli we-CYP1A1 [1].Ukwelashwa kwe-Galangin kuvimbele ukwanda kwamaseli kanye ne-autophagy (130) μ M) kanye ne-apoptosis (370 μ M) Ikakhulukazi, ukwelashwa kwe-galangin kumaseli e-HepG2 kubangele (1) ukunqwabelana kwama-autophagosomes, (2) anda amazinga we-microtubule ehambisana neketango lokukhanya lamaprotheni. 3, kanye (3) nephesenti elikhuphukile lamaseli anama-vacuoles. Isisho se-P53 siphinde sanyuka. I-autophagy eyenziwe i-Galangin yancishiswa ngokuvimbela i-p53 kumaseli e-HepG2, futhi ukuvezwa ngokweqile kwe-p53 kumaseli e-Hep3B kubuyisele amaphesenti aphezulu ama-vacuoles eseli adangwe i-galangin kuya kumazinga ajwayelekile. [2].
Ukuhlolwa Kweseli
Amangqamuzana (5.0 × 103) ajovwe futhi aphathwe ngokugxila okuhlukene kwe-galangin kumapuleti wemithombo engama-96 ngezikhathi ezahlukene.Ngokungeza u-10 μ L wesixazululo se-MTT esingu-5 mg / ml ukuze kutholakale inani lamaseli aphilayo emthonjeni ngamunye.Ngemva kokufukamela ku-37 ℃ amahora angu-4, amaseli ahlakazwa ngesisombululo esingu-100% esiqukethe u-20% we-SDS kanye nesisombululo se-dimethylformamide μ L esingu-50%.Ukuminyana kokubona kulinganiswa kusetshenziswa i-spectrophotometer ye-varioskan flash reader kubude begagasi bokuhlola obungu-570 nm kanye nobude bereferensi obungu-630 nm.
