I-Glycyrrhizin, i-Liquiritin;I-Liquiritoside;I-Likviritin;I-Liquiritoside Cas No.551-15-5
Isingeniso esifushane
I-Glycyrrhizin, eyaziwa nangokuthi i-Liquiritin.I-Licorice isitshalo se-Glycyrrhiza kuLeguminosae.Izimpande neziqu zawo amakhambi avamile aseShayina.
Umuthi usatshalaliswa kabanzi eNyakatho-mpumalanga yeChina, eXinjiang, eYunnan, e-Inner Mongolia, e-Anhui nakwezinye izindawo.I-Shennong materia medica classic iklelisa njengebanga eliphezulu, ithi "lobu tshani buyinkosi yayo yonke imithi, futhi bambalwa abangabusebenzisi".I-Licorice inezingxenye eziyinkimbinkimbi, ikakhulukazi ezihlanganisa ama-triterpenoids, ama-flavonoid nama-coumarins.I-Flavonoids iwuhlobo lwezingxenye ze-bioactive ezitholakala ku-licorice ekhishwe.Izingxenye zayo zamakhemikhali okwelapha ikakhulukazi zifaka i-glycyrrhizin, isoglycyrrhizin, glycyrrhizin, isoglycyrrhizin, neoglycyrrhizin, njll. Eminyakeni yamuva nje, kuye kwaba nemibiko mayelana nokukhishwa kwe-radical yamahhala, i-antioxidant, anti-cancer kanye nemiphumela ephikisana ne-mutagenic ye-licorice flavonoids ekhaya naphesheya.
AmakhemikhaliName:4H-1-Benzopyran-4-one, 2- [4-(β-D-glucopyranosyloxy) phenyl]-2, 3-dihydro-7-hydroxy-, (S)
PhysicalPimpahla:I-Monohydrate (dilute ethanol noma amanzi), indawo yokuncibilika: 212 ~ 213 ° ℃.
Isenzo se-Pharmacological
Ubuthi: Akukho
Ukusabela okubi: akwaziwa
Umthombo wesithako: i-legume Glycyrrhiza glabra L. impande, Glycyrrhiza uralensis Fisch Root.
Ukukhishwa kwe-Glycyrrhizin
Ukwelashwa Kwangaphambili Kwezinto Ezingavuthiwe ze-Licorice
Ukwakhiwa kwamakhemikhali we-licorice impahla eluhlaza kuyinkimbinkimbi kakhulu.Ukuze uthole umphumela ongcono wokuhlukanisa, wehlise ukungcoliswa kokungcola kukholamu ye-chromatographic yokulungiselela, futhi uthuthukise okuqukethwe kwe-glycyrrhizin kumpahla eluhlaza yomjovo, indlela yokukhipha yasetshenziswa ukuze kulungiswe kuqala impahla eluhlaza.Kala i-4G hay ngebhalansi ye-elekthronikhi bese uyifake ebhekhani.Kala ngokunembile u-100ml wamanzi acwengiwe ngesilinda sokulinganisa bese uwathela ebhekhani ukuze ahlakazwe.I-Ultrasonic imizuzu engaba ngu-15, futhi uhlale uvuselela ngenduku yengilazi ukusheshisa ukuqedwa.Bese ufaka ibhikha kubhavu we-thermostatic ongu-90 ℃ bese uyifudumeza amahora angu-2, bese uyishisa ukuze ihlungwe.Ngemva kokwengeza i-filtrate ku-n-butanol solvent nokuma imizuzu embalwa, iningi lama-glycosides liyancibilika ku-n-butanol solvent, bese likhipha ukukhishwa kwesibili, likhiphe inani elincane lama-glycosides asele emanzini, futhi ekugcineni ahlanganise futhi agxilise i-n- Isixazululo se-butanol esitholwe ngokukhishwa kwesibili kwe-chromatography nokuhlanzwa.
Ukuhlanzwa Kwe-Glycyrrhizin Nge-Chromatography
Thatha u-10 ml womkhiqizo okhishwe ngenhla njenge-spare raw material, qala iphampu, setha izinga lokugeleza libe ku-25 ml/min, bese uletha okuluhlaza ku-500 mm ngesigaba seselula (methanol: amanzi = 1:4) × Ikholomu yokulungiselela engu-40 mm, qoqa ingxenye yomkhiqizo we-hay glucoside ngokwesimo esiphezulu: ingxenye yehora elingu-1 iqoqwa ndawonye njengengxenyana yangaphambi kokungcola, bese ishintsha ukugeleza.Isibonelo, geza ikholomu ngengxube engu-50% ye-methanol namanzi, xhuma umkhiqizo njalo emizuzwini engu-20, bese ugxilisa ibhodlela ngalinye lomkhiqizo ngokuhwamuka okujikelezayo, futhi uthathe u-20 µ L wokuhlaziywa kwe-chromatographic ye-HPLC, Kuze kube yilapho kutholwa ithagethi.Izimo zokuthola i-HPLC zazimi kanje: isigaba seselula: i-methanol: amanzi = 3.5: 6.5;Isigaba sokumisa: i-silica gel carbon 18;Ikholomu ye-Chromatographic: 450 mm × 4.6 mm; Izinga lokugeleza: 1 ml / min;Ubude begagasi bokutholwa: 254nm.Okuqukethwe kwe-glycyrrhizin ebhodleleni lesibili kuphakeme kakhulu phakathi kwemikhiqizo etholwe njalo ngemizuzu engama-20
Ukuhlanzwa Kwe-Glycyrrhizin Nge-Rechromatography
Njengoba okuqukethwe kwe-glycyrrhizin ngemva kokuhlanzwa okuyinhloko kwe-chromatographic akuphakeme, indlela efanayo ikhethiwe.Thatha u-10 ml womkhiqizo ohlanziwe ngenhla njengento eluhlaza ebekwe eceleni, izinga lokugeleza lingu-25 ml / min, bese uletha ibhodlela lesibili lomkhiqizo libe ngu-500 mm ngesigaba seselula (i-methanol: amanzi = 2:5) × Ku-20 mm } ikholomu ye-chromatographic, qoqa i-distillate yomkhiqizo we-hay glycoside ngokuya ngesimo esiphezulu: xhuma umkhiqizo njalo emizuzwini emi-4, bese ugxilisa ibhodlela ngalinye lomkhiqizo ngokuhwamuka okujikelezayo, futhi usebenzise umugqa ofanayo wokuthola ngenhla ukuze uhlaziye i-chromatographic ye-HPLC kuze kungabikho okuhlosiwe. .Ngemuva kokuhlaziywa, kwatholakala ukuthi okuqukethwe kwe-glycyrrhizin ebhodleleni lesithupha kwakuphakeme kakhulu phakathi kwemikhiqizo etholwe njalo emizuzwini emi-4, lapho isikhathi sokugcina sasingu-5.898 min njengesiqongo esihlosiwe, futhi okuqukethwe kufinyelele cishe ku-40% ngendlela yokujwayelekile yendawo. .
Thumela Ukwelashwa Kwemikhiqizo
Umkhiqizo oqoqiwe uhlanjululwa ngaphansi kwengcindezi encishisiwe ku-evaporator ejikelezayo ku-70 ℃.Ngemva kokuba isincibilikisi sesihwamukile, ncibilikisa umkhiqizo oqinile eflaskini engezansi eyindilinga ngenani elincane le-methanol, bese ucwebezela eshubhuni lokuhlola ekamelweni lokushisa kuze kuvele amakristalu amhlophe ayimbudumbudu [2].
