Imikhiqizo

Ithagethi:JNK1 jnk2 NF- κ B

Isifundo se-in vitro:I-isovitexin ivimbela ukulimala kwe-LPS okwenziwe nge-oxidative ngokuvimbela ukukhiqizwa kwe-ROS ye-intracellular, futhi yenza buthaka umphumela we-H2O2 ekusebenzeni kweseli.Iqukethe i-LPS (2 μ Isovitexin (0-100 g / ml) μ G / ml) yayingenayo i-cytotoxicity kumaseli aluhlaza angama-264.7, kodwa i-isovitexin engu-200 μ G / ml ibonise i-cytotoxicity ebalulekile.I-Isovitexin (25,50) μ G / ml) ivinjiwe i-LPS ifake i-TNF- α， Ukukhuphuka kwamazinga e-IL-6, iNOS ne-COX-2.I-Isovitexin (25,50) μ G / ml) iphinde ivimbele I kumaseli aluhlaza angama-264.7 κ B α Phosphorylation kanye nokuwohloka, okuhambisana nomthelela we-JNK1 / 2 inhibitor [1].

Izifundo ze-in vivo:I-isovitexin (i-50 kanye ne-100 mg / kg, i-IP) ibangele izinguquko ezimbi kakhulu ze-histopathological ezigabeni zamaphaphu futhi yehlisa inani lamaseli avuthayo kumagundane e-LPS.I-Heteroeosinophils (i-50 ne-100 mg / kg, i-IP) yehla i-TNF nge- α Futhi ukukhiqizwa kwe-IL-6, ukukhiqizwa kwe-ROS, okuqukethwe kwe-MPO ne-MDA, ukwanda kwe-SOD ne-GSH, nokuvimbela i-LPS eyenza ukuvuvukala nokucindezeleka okwenziwe nge-oxidative ku-LPS eyenga amagundane e-Ali.Futhi kuvimbela ngempumelelo ukubonakaliswa kwamaprotheni e-iNOS ne-COX-2 [1].I-Isovitexin (25,50, 100 mg / kg) yehlise izinga lokusinda lokulimala kwesibindi okudalwe yi-LPS/D-gal kumagundane ngendlela encike kumthamo.I-Isovitexin iphinde yavimbela i-NF- κ B futhi ilawula i-LPS / D-gal eyenziwe nge-Nrf2 kanye ne-HO-1 kumagundane [2].

Ukuhlolwa kweseli:ukusebenza kweseli kwanqunywa ukuhlolwa kwe-MTT.Amangqamuzana angama-264.7 aluhlaza afakwe emapuletini angu-96 (1) × 104 amaseli / kahle) kanye nokugxila okuhlukahlukene kwe-isovitexin (ukuhlushwa kokugcina: 0-200) μ G / ml) kanye ne-LPS (2 μ G / ml) amahora angu-24.Ngaphezu kwalokho, ukusetshenziswa IV (25 noma 50 μ G / ml) pretreated amaseli ihora eli-1, bese H 2O 2 (300) yengezwa μ M）。 Ngemuva kwamahora angama-24, i-MTT (5 mg / ml) yengezwa amaseli bese efukanyelwa amahora angu-4 [1].

Ukuhlolwa kwezilwane:amagundane [1] ukuze kusungulwe imodeli ye-Ali, amagundane ahlukaniswa ngokungahleliwe abe amaqembu e-6: ukulawula (usawoti), kuphela i-isovitexin (100 mg / kg, ehlakazwe ku-0.5% DMSO), kuphela i-LPS (0.5 mg / kg, encibilikiswe ku-saline ), LPS (0.5 mg / kg) + isovitexin (50 noma 100 mg / kg) kanye LPS (0.5 mg / kg) + dexamethasone (DEX, 5 mg / kg, encibilike saline).I-isovitexin noma i-DEX (5 mg / kg) i-isovitexin yasetshenziswa.Ngemva kokuchayeka ku-isovitexin noma i-DEX ngehora le-1, amagundane abulawa izinzwa nge-ether futhi i-LPS yanikezwa nge-intranasally (in) ukuze yenze ukulimala kwamaphaphu.Izilwane zahlukunyezwa emahoreni ayi-12 ngemuva kokuphathwa kwe-LPS.Ngakho-ke, i-bronchoalveolar lavage fluid (BALF) namasampula ezicubu zamaphaphu avunwa ukukala amazinga e-cytokine;Isizukulwane se-ROS;Imisebenzi ye-SOD, GSH, MDA kanye ne-MPO;Futhi inkulumo ye-COX-2, iNOS, i-HO-1 ne-Nrf2 amaprotheni [1].