I-Paeoniflorin CAS No. 23180-57-6
Ulwazi Olubalulekile
Eyaziwa nangokuthi i-paeoniflorin, iyi-pinane monoterpene bitter glycoside ehlukanisiwe ne-peony ebomvu ne-peony emhlophe.I-hygroscopic amorphous powder.Itholakala ezimpandeni zePaeonia, peony, peony onsomi nezinye izitshalo zeRanunculaceae.Ubuthi bale crystal buphansi kakhulu.
[igama lekhemikhali]5beta--[(Benzoyloxy)methyl]tetrahydro-5-hydroxy-2-methyl-2,5-methano-1H-3,4-dioxacyclobuta[cd]pentalen-1alpha(2H)-yl-beta-D-glucopyranoside
[ifomula yamangqamuzana]I-C23H28O11
【I-CASCha】23180-57-6
Ubumsulwa: ngaphezu kwama-98%, indlela yokuthola: HPLC.
[umthombo]izimpande ze-Paeonia albiflora pall, P. suffrsticosa Andr, P. delarayi Franch, isitshalo se-Ranunculaceae, okuqukethwe kwe-radix paeoniae Rubr kuphakeme kakhulu.
[Ukucaciswa]10%, 20%, 30%, 50%, 90%, 98%
[AesebenzayoIisithako ] Ingqikithi ye-glucosides ye-Paeonia (TGP) igama elijwayelekile le-paeoniflorin, i-hydroxy paeoniflorin, i-paeoniflorin, i-albiflorin ne-benzoyl paeoniflorin, ebizwa ngokuthi i-TGP ngamafuphi.
Izakhiwo zomzimba namakhemikhali
I-hygroscopic amorphous Tan powder (90% ivaliwe impushana emhlophe)[ α] 16D-12.8。 (C = 4.6, i-methanol), i-tetraacetate iyikristalu ye-acicular engenambala, indawo encibilikayo: 196 ℃.I-Paeoniflorin izinzile endaweni ene-asidi (pH 2 ~ 6) futhi ayizinzile endaweni ene-alkali.
Ukunqunywa kokuqukethwe
Ngokuvamile, indlela yoku-1 kanye nendlela yesi-2 nayo ingasetshenziselwa ukutholwa.Indlela 1 isetshenziswa kangcono ekukhiqizeni okuqukethwe okuphezulu, okungasiza ukucubungula abasebenzi bahlulele kangcono ukuhlanzeka kwemikhiqizo.Into eyisithenjwa kulula ukubola ngemva kokuhlakazwa.
1.Kwanqunywa ukusebenza okuphezulu kwe-chromatography ye-liquid (Isithasiselo VI d).Izimo ze-chromatographic nokufaneleka kwesistimu kwahlolwa nge-Octadecyl silane bonded silica gel njengesigcwalisi;I-Acetonitrile-0.1% isisombululo se-phosphoric acid (14:86) sisetshenziswe njengesigaba seselula;Ubude bokutholwa bungama-230nm.Inombolo yamapuleti etiyetha abalwe ngokusho kwe-paeoniflorin peak akufanele ibe ngaphansi kuka-2000. Ukulungiswa kwesixazululo sereferensi: kala ngokunembile inani elifanele lesixazululo sereferensi se-paeoniflorin bese wengeza i-methanol ukuze ulungise i-paeoniflorin engu-60% ngesisombululo se-1ml μ G.
2.Ukuthuthukisa indlela yokuzimisela ye-paeoniflorin ku-Radix Paeoniae Alba.Izindlela: izindlela ze-Chinese Pharmacopoeia kanye nezindlela ezithuthukisiwe zaqhathaniswa.Isigaba esihambayo bekungamanzi e-methanol (30:70) kanti ubude begagasi bokutholwa babungu-230nm.umphumela;Ubudlelwano bomugqa bale ndlela buhle (r = 0.9995).Isilinganiso sokululama singu-101.518% kanti i-RSD ingu-1.682%.Isiphetho: indlela ethuthukisiwe ilula futhi inembile, enganciphisa ubuthi bezincibilikisi eziphilayo kubantu kanye nokungcoliswa kwemvelo, futhi inikeze isisekelo sokubhekisela ekunqumeni kwe-paeoniflorin ekusebenzeni.
Indlela Yokunquma
Ukunqunywa kwe-paeoniflorin nge-HPLC
Ububanzi besicelo:le ndlela isebenzisa i-HPLC ukunquma okuqukethwe kwe-paeoniflorin kumaphilisi e-Guizhi Fuling.
Indlela ifanele iphilisi le-Guizhi Fuling.
Umgomo wendlela:faka isampula yokuhlola eflaskini eyi-conical, engeza inani elifanele le-ethanol ehlanjululwe ukuze kukhishwe i-ultrasonic, ipholise, inyakazise kahle, ihlunge, i-filtrate ingena ku-chromatograph ye-liquid esebenza kahle ngokuhlukaniswa kwe-chromatographic, sebenzisa umtshina we-ultraviolet absorption ukuze uthole inani lokumuncwa le-paeoniflorin kubude begagasi obungu-230nm, futhi ubale okuqukethwe kwayo.
