I-Salvianolic acid B / Lithospermic acid B Lithospermate-B CAS No.115939-25-8
Ulwazi Olubalulekile
I-Salvianolic acid B iyi-condensation yama-molecule amathathu e-Danshensu kanye ne-molecule eyodwa ye-caffeic acid.Ingenye ye-salvianolic acid efundwa kakhulu.Inemiphumela ebalulekile ye-pharmacological enhliziyweni, ubuchopho, isibindi, izinso nezinye izitho.Lo mkhiqizo unemiphumela yokukhuthaza ukujikeleza kwegazi nokususa i-stasis yegazi, ukudonsa ama-meridians kanye nokwenza kusebenze izibambiso.Isetshenziswa kakhulu ukwelapha i-ischemic stroke ebangelwa i-blood stasis evimba ama-meridians, njengokuba ndikindiki kwengxenye yomzimba nezitho, ubuthakathaka, ubuhlungu benkontileka, ukwehluleka kwemoto, ukuchezuka komlomo namehlo, njll.
Isibizo:i-salvianolic acid B, i-salvianolic acid B, i-salvianolic acid B
Igama lesiNgisi:i-salvianolic acid B
Ifomula ye-molecular:c36h30o16
Isisindo samangqamuzana:718.62
Inombolo ye-CAS:115939-25-8
Indlela yokuthola:I-HPLC ≥ 98%
Imininingwane:10mg, 20mg, 100mg, 500mg, 1g (ingapakishwa ngokwezidingo zekhasimende)
Umsebenzi kanye nokusetshenziswa:Lo mkhiqizo usetshenziselwa ukunquma okuqukethwe.
Izakhiwo zomzimba namakhemikhali
Izakhiwo:umkhiqizo uyimpushana emhlophe.
Ukunambitheka kubaba kancane futhi ku-astringent, kunezinto eziheha umswakama.Incibilika emanzini, i-ethanol ne-methanol.
I-Salvianolic acid B yenziwa ukufingqa kwama-molecule angu-3 e-salvianolic acid kanye ne-molecule engu-1 ye-caffeic acid.Inamaqembu amabili e-carboxyl futhi ikhona ngendlela yosawoti abahlukene (K +, Ca2 +, Na +, NH4 +, njll.).Enqubweni ye-Decoction kanye nokuhlushwa, ingxenye encane ye-salvianolic acid B i-hydrolyzed ibe yi-oxalic acid ensomi kanye ne-salvianolic acid, futhi ingxenye ye-salvianolic acid B iba yi-rosmarinic acid ngaphansi kwezimo ze-acidic;I-Salvianolic acid A no-C ingaba yi-tautomeric kusixazululo.
Imininingwane
＞5%,＞10%,＞50%,＞70%,＞90%,98%
Inqubo yokukhipha
I-Radix Salviae Miltiorrhizae yachotshozwa, yafakwa ethangini lokukhipha, yacwiliswa izikhathi ezingu-8 inani le-hydrochloric acid engu-0.01mol/l ngobusuku obubodwa, yabe isicwiliswa ngamanzi aphindwe izikhathi ezingu-14.Isixazululo esikhishwe ngama-percolated sihlanzwa ngekholomu ye-AB-8 macroporous resin.Okokuqala, i-elute nge-0.01mol/l hydrochloric acid ukususa ukungcola okungakhangisiwe, bese i-ethanol engu-25% ukuze isuse ukungcola okuphezulu kwe-polar.Okokugcina, gxilisa i-ethanol engu-40% ngaphansi kwengcindezi encishisiwe yokuthola i-ethanol nokomisa ukuze uthole isamba se-Salvia miltiorrhiza phenolic acid enobumsulwa obungaphezu kuka-80%.
Khomba
Thatha u-1g womkhiqizo, uwugaye, wengeze u-5ml we-ethanol, ugovuze ngokugcwele, uhlunga, uthathe amaconsi ambalwa e-filtrate, faka ichashaza emgqeni wephepha lokuhlunga, uyomise, ubheke ngaphansi kwesibani se-ultraviolet (365nm), ubonise blue- i-gray fluorescence, ilengisa iphepha lokuhlunga ebhodleleni lesixazululo se-ammonia eligxilile (ungaxhumani noketshezi olumanzi), likhiphe ngemva kwemizuzu engu-20, libheke ngaphansi kwesibani se-ultraviolet (365nm), bonisa i-fluorescence eluhlaza okwesibhakabhaka.
I-Acidity:thatha isixazululo esinamanzi ngaphansi kwento yokucacisa, futhi inani le-pH lizoba ngu-2.0 ~ 4.0 (isithasiselo soHlelo lwe-Chinese Pharmacopoeia 1977).
Ukunqunywa kokuqukethwe
Kunqunywe ukusebenza okuphezulu kwe-chromatography yoketshezi (Isithasiselo VI D, Umqulu I, i-Chinese Pharmacopoeia, 2000 EDITION).
Ijeli ye-silica ehlanganisiwe ye-Octadecyl silane isetshenziswe njengesigcwalisi ezimeni ze-chromatographic kanye nokuhlolwa kokusebenza kwesistimu;I-Methanol acetonitrile formic acid amanzi (30:10:1:59) kwakuyisigaba esihambayo;Ubude bokutholwa bebungu-286 nm.Inani lamapuleti etiyori abalwe ngokwenani eliphakeme le-salvianolic acid B akufanele libe ngaphansi kuka-2000.
Ukulungiswa kwesixazululo sereferensi kala ngokunembile inani elifanele lesixazululo sereferensi se-salvianolic acid B bese wengeza amanzi ukuze alenze liqukathe u-10% ngesisombululo se-1ml μ G ngasinye.
Ukulungiswa kwesixazululo sokuhlola kuthatha cishe u-0.2g womkhiqizo, ukale kahle, uwubeke ebhodleleni lokulinganisa elingu-50ml, engeza inani elifanele le-methanol, i-sonicate imizuzu engu-20, uyipholise, engeza amanzi esikalini, inyakazise kahle, isihlungi. it, ulinganise ngokunembile u-1ml we-filtrate eqhubekayo, uyifake ebhodleleni lokulinganisa elingu-25ml, engeza amanzi esikalini, unyakazise kahle.
Indlela yokunquma ithatha ngokunembile i-20% yesisombululo sokulawula kanye ne-20% yesisombululo sokuhlola μ l.Yijove ku-chromatograph ewuketshezi ukuze unqume.
Ukusebenza Kwemithi
I-Salvianolic acid B iyi-condensation yama-molecule amathathu e-Danshensu kanye ne-molecule eyodwa ye-caffeic acid.Ingenye ye-salvianolic acid efundwa kakhulu.Inemiphumela ebalulekile ye-pharmacological enhliziyweni, ubuchopho, isibindi, izinso nezinye izitho.
I-Antioxidant
I-Salvianolic acid B inomphumela onamandla we-antioxidant.Ukuhlolwa kwe-vivo ne-in vitro kubonisa ukuthi i-salvianolic acid B ingasusa ama-radicals angenawo umoya-mpilo futhi ivimbele i-lipid peroxidation.Ukuqina kwayo kwesenzo kuphezulu kunovithamini C, uvithamini E kanye nemannitol.Ingenye yemikhiqizo yemvelo eyaziwayo enomphumela onamandla we-antioxidant Izifundo ze-Pharmacological zibonisa ukuthi i-salvianolic acid yomjovo inomphumela osobala we-antioxidant, ivimbela ukuhlangana kweplatelet kanye ne-thrombosis, futhi ingandisa isikhathi sokusinda sezilwane ngaphansi kwe-hypoxia.Imiphumela yabonisa ukuthi i-salvianolic acid yomjovo (60 ~ 15mg / kg) ingathuthukisa kakhulu ukushoda kwezinzwa kumagundane anokulimala kwe-cerebral ischemia-reperfusion, ukuthuthukisa ukuphazamiseka kokuziphatha futhi kunciphise kakhulu indawo ye-cerebral infarction.Kwakukhona umehluko omkhulu phakathi kwemithamo ephezulu nephakathi (60 kanye 30mg / kg);I-Salvianolic acid yomjovo ingathuthukisa kakhulu umonakalo wezinzwa obangelwa i-FeCl3 eyenza i-cerebral ischemia kumagundane ku-1, i-2 namahora angu-24 ngemva kokuphathwa, okubonakaliswa ekuthuthukiseni ukuphazamiseka kokuziphatha kanye nokunciphisa indawo ye-cerebral infarction;I-Salvianolic acid 40 mg / kg yomjovo ivimbele ngokuphawulekayo ukuhlanganiswa kwamaplatelet onogwaja okubangelwa i-ADP, i-arachidonic acid ne-collagen, futhi amazinga okuvimbela ayengu-81.5%, 76.7% no-68.9% ngokulandelana.I-Salvianolic acid 60 kanye ne-30mg / kg yomjovo ivimbele kakhulu i-thrombosis kumagundane;I-Salvianolic acid 60 kanye ne-30mg / kg yomjovo yandisa kakhulu isikhathi sokusinda samagundane ngaphansi kwe-hypoxia.
Isicelo somtholampilo
Lo mkhiqizo unemiphumela yokukhuthaza ukujikeleza kwegazi nokususa i-stasis yegazi, ukudonsa ama-meridians kanye nokwenza kusebenze izibambiso.Isetshenziswa kakhulu ukwelapha i-ischemic stroke ebangelwa i-blood stasis evimba ama-meridians, njengokuba ndikindiki kwengxenye yomzimba nezitho, ubuthakathaka, ubuhlungu benkontileka, ukwehluleka kwemoto, ukuchezuka komlomo namehlo, njll.
Isitolo
Endaweni epholile neyomile.
Isikhathi Sokusebenza
Iminyaka emibili.
Indlela Yesitoreji
I-2-8 ° C, igcinwe endaweni epholile neyomile futhi kude nokukhanya.
Izindaba Ezidinga Ukunakwa
Umkhiqizo kufanele ugcinwe ekushiseni okuphansi.Uma ivezwa emoyeni isikhathi eside, okuqukethwe kuzoncishiswa.
